ArticleBMC oral health2021
Comparative evaluation of the effect of cold ceramic and MTA-Angelus on cell viability, attachment and differentiation of dental pulp stem cells and periodontal ligament fibroblasts: an in vitro study.
Article in BMC oral health, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
- Clinical and Radiographic Outcomes Following Unintentional Extrusion of Cold Ceramic: A Case Series of Three Patients.Clinical case reports · 2026Article
- Cytotoxicity and Bioactivity of Five Hydraulic Silicate Cements in Human Dental Pulp Cells at Different Setting Stages.International dental journal · 2026Article
- Pulpotomy for an Immature Permanent Tooth With Symptomatic Irreversible Pulpitis Using Cold Ceramic.Case reports in dentistry · 2026Article
- Regenerative Endodontic Treatment in an Immature Permanent Tooth With Necrotic Pulp and Periradicular Lesion.Clinical case reports · 2025Article
- Article
- Nonsurgical Management of Perforating Internal Root Resorption in Maxillary Invaginated Lateral Incisor Using Cold Ceramic.Clinical case reports · 2025Article
- Cytotoxicity and proliferation effects of cold ceramic on stem cells from human exfoliated deciduous teeth compared to MTA: an in vitro study.BMC oral health · 2025Article
- Cold Ceramic for Repairing Root Perforations: A Case Report.Clinical case reports · 2025Article
- In vitro comparison of the marginal adaptation of cold ceramic sealer with the single-cone obturation technique versus AH-26 sealer with the lateral compaction technique in single-canal teeth.BMC oral health · 2025Article
- Cell Biological and Antibacterial Evaluation of a New Approach to Zirconia Implant Surfaces Modified with MTA.Biomimetics (Basel, Switzerland) · 2024Article
- Conservative management of an advanced external cervical resorption with internal approach using bio-ceramic materials: A case report.Clinical case reports · 2024Article
- Effect of blood contamination on marginal adaptation of cold ceramic and MTA angelus: a scanning electron microscopic study.BMC oral health · 2023Article
- Expression of Interleukin-1β and Histological Changes of the Three-Dimensional Oral Mucosal Model in Response to Yttria-Stabilized Nanozirconia.Materials (Basel, Switzerland) · 2023Article
- Biological Evaluation of Zinc Phosphate Cement for Potential Bone Contact Applications.Biomedicines · 2023Article
- Nonsurgical Endodontic Management of Large Periapical Lesion with Cold Ceramic: A Literature Review and Case Series.Iranian endodontic journal · 2023Article
- Comparative cytocompatibility of the new calcium silicate-based cement NeoPutty versus NeoMTA Plus and MTA on human dental pulp cells: an in vitro study.Clinical oral investigations · 2022Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBiocompatibility and induction of mineralized tissue formation are the properties expected from a material used in vital pulp therapy and repair of perforations. Cold ceramic (SJM, Iran; CC) is a newly introduced calcium silicate-based cement for above mentioned therapeutic applications. This in-vitro study aimed to compare the effect of CC and White MTA-Angelus (MTA) on cell viability, attachment, odontogenic differentiation, and calcification potential of human dental pulp stem cells (DPSCs) and periodontal ligament fibroblasts (PDLFs).
methodsCell viability of DPSCs and PDLFs was assessed using MTT on days 1, 3, 7, and 14 (n = 9) in contact with freshly mixed and set states of CC and MTA. Field emission scanning electron micrographs (FESEM) were taken to evaluate cell-bioceramic interaction (n = 6). Gene expression levels of osteo/odontogenic markers (Dentin sialophosphoprotein, Dentin matrix protein 1, Collagen type I alpha 1, and Alkaline phosphatase (DSPP, DMP1, COL 1A1, and ALP, respectively) (n = 8) were assessed using qrt-PCR. ALP enzymatic activity was evaluated to assess the mineralization potential. A two-way ANOVA test was applied, and p < 0.05 was considered to be statistically significant.
resultsThe effect of freshly mixed and set MTA and CC on the survival of DPSCs and PDLFs in all study groups was statistically similar and comparable to the positive control group (p > 0.05); the only exception was for the viability of PDLFs in contact with freshly mixed cements on day 1, showing a more significant cytotoxic effect compared to the control and the set state of materials (p < 0.05). PDLFs attached well on CC and MTA. The spread and pseudopodium formation of the cells increased on both samples from day 1 to day 14. Contact of MTA and CC with DPSCs similarly increased expression of all dentinogenesis markers studied on days 7 and 14 compared to the control group (p < 0.001), except for DSPP expression on day 7 (p = 0.46 and p = 0.99 for MTA and CC, respectively).
conclusionsWithin the limitation of this in-vitro study, cold ceramic and MTA-Angelus showed high biocompatibility and induced increased expression of osteo/dentinogenic markers. Therefore, cold ceramic can be a suitable material for vital pulp therapy and the repair of root perforations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.