ArticleEuropean journal of trauma and emergency surgery : official publication of the European Trauma Society2022
Is the bioactivity of induced membranes time dependent?
Article in European journal of trauma and emergency surgery : official publication of the European Trauma Society, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.
- Bone defect treatment: does the type and properties of the spacer affect the induction of Masquelet membrane? Evidence today.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2022Pooled it
- Naringin targets TGF-β1-mediated angiogenesis to enhance the osteogenic effect of induced membrane.Bone & joint research · 2026Article
- [Mechanism of actions and reflections of Masquelet induced membrane technique in diabetic wound].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2026Review
- The masquelet technique for severe open fractures: current insights.Journal of orthopaedic surgery and research · 2026Review
- Cell Supported Single Membrane Technique for the Treatment of Large Bone Defects: Depletion of CD8Cells · 2026Article
- Clinical efficacy of Masquelet technique in the treatment of tibial and fibular bone defects caused by severe gunshot wounds.Frontiers in medicine · 2026Article
- Modified induced membrane technique using a non-vascularized fibular graft for chronic osteomyelitis of the metatarsal bone: A case report.Trauma case reports · 2025Article
- A review of the Masquelet technique in the treatment of lower limb critical-size bone defects.Annals of the Royal College of Surgeons of England · 2025Review
- Lithium chloride modulates macrophage polarization and enhances bone defect healing in the induced membrane technique by activation of Wnt/β-catenin signaling.Frontiers in pharmacology · 2025Article
- Human Acellular Collagen Matrices-Clinical Opportunities in Tissue Replacement.International journal of molecular sciences · 2024Review
- The Impact of Defect Size on Bone Healing in Critical-Size Bone Defects Investigated on a Rat Femur Defect Model Comparing Two Treatment Methods.Bioengineering (Basel, Switzerland) · 2024Article
- Enhancement of a one-step membrane technique for the treatment of large bone defects by pre-seeding the membrane with CD8 lymphocyte depleted bone marrow mononuclear cells in a rat femoral defect model.Frontiers in immunology · 2024Article
- Measuring Bone Healing: Parameters and Scores in Comparison.Bioengineering (Basel, Switzerland) · 2023Article
- Varying degrees of spontaneous osteogenesis of Masquelet's induced membrane: experimental and clinical observations.BMC musculoskeletal disorders · 2023Article
- Article
- [Biological reconstruction of large bone defects : Masquelet technique and new procedures].Unfallchirurgie (Heidelberg, Germany) · 2023Review
- Masquelet technique in military practice: specificities and future directions for combat-related bone defect reconstruction.Military Medical Research · 2022Review
- Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe induced membrane technique (IMT) is a two-stage surgical procedure for reconstruction of bone defects. Bone grafting (second stage of IMT) is recommend after 4-8 weeks assuming the highest bioactivity of IMs. However, larger studies concerning the biology and maturation of IMs and a potential time dependency of the bioactivity are missing. Therefore, aim of this study was the time-dependent structural and cellular characterization of cement spacer IMs concomitantly to an analysis of membrane bioactivity.
methodsIMs from 60 patients (35-82 years) were obtained at different maturation stages (1-16 weeks). IMs were studied by histology and co-culture with mesenchymal stem cells (MSC). IM lysates were analyzed by ELISA and protein microarray.
resultsIncreasing vascularization and fibrosis were found in membranes older than 4 and 7 weeks, respectively. MSC grew out from all membranes and all membranes enhanced proliferation of cultured MSC. Osteocalcin and osteopontin (in membrane lysates or induced in MSC by membrane tissue) were found over all time points without significant differences. In contrast to alkaline phosphatase activity, increasing levels of osteoprotegerin were found in membranes.
conclusionThe histological structure of IMs changes during growth and maturation, however, biologically active MSC and factors related to osteogenesis are found over all time points with minor changes. Thus, membranes older than 8 weeks exert regenerative capacities comparable to the younger ones. The postulated narrow time frame of 4-8 weeks until bone grafting can be questioned and surgeons may choose timing for the second operation more independently and based on other clinical factors.
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