ArticleGenome biology2021
Single-cell characterization of CRISPR-modified transcript isoforms with nanopore sequencing.
Article in Genome biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Genetic demultiplexing and transcript start site identification from nanopore sequencing of 10x Genomics multiome libraries.bioRxiv : the preprint server for biology · 2026Article
- Proteoform medicine: characterizing and targeting protein forms in human disease.Nature reviews. Genetics · 2026Review
- Review
- Single cell and spatial alternative splicing analysis with Nanopore long read sequencing.Nature communications · 2025Article
- Direct measurement of engineered cancer mutations and their transcriptional phenotypes in single cells.Nature biotechnology · 2024Article
- Advances in single-cell long-read sequencing technologies.NAR genomics and bioinformatics · 2024Article
- Recent advances in CRISPR-based functional genomics for the study of disease-associated genetic variants.Experimental & molecular medicine · 2024Review
- Next-generation forward genetic screens: uniting high-throughput perturbations with single-cell analysis.Trends in genetics : TIG · 2024Review
- Single-cell multi-gene identification of somatic mutations and gene rearrangements in cancer.NAR cancer · 2023Article
- Massively Parallel CRISPR-Based Genetic Perturbation Screening at Single-Cell Resolution.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- CellMarker 2.0: an updated database of manually curated cell markers in human/mouse and web tools based on scRNA-seq data.Nucleic acids research · 2023Article
- Single-cell characterization of CRISPR-modified transcript isoforms with nanopore sequencing.Genome biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We developed a single-cell approach to detect CRISPR-modified mRNA transcript structures. This method assesses how genetic variants at splicing sites and splicing factors contribute to alternative mRNA isoforms. We determine how alternative splicing is regulated by editing target exon-intron segments or splicing factors by CRISPR-Cas9 and their consequences on transcriptome profile. Our method combines long-read sequencing to characterize the transcript structure and short-read sequencing to match the single-cell gene expression profiles and gRNA sequence and therefore provides targeted genomic edits and transcript isoform structure detection at single-cell resolution.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.