Evidence map›Paper›PMID 34871326›Full record

ArticlePLoS pathogens2021

RSK1 SUMOylation is required for KSHV lytic replication.

Zhenshan Liu, Chengrong Liu, Xin Wang, Wenwei Li, Jingfan Zhou, Peixian Dong, Maggie Z X Xiao, Chunxia Wang, Yucai Zhang, Joyce Fu and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS pathogens, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.6field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Zhenshan LiuResearch Center of Translational Medicine, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Chengrong LiuResearch Center of Translational Medicine, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Xin WangResearch Center of Translational Medicine, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Wenwei LiDepartment of Biological Science, Florida State University, Tallahassee, Flordia, United States of America.ORCID 0000-0003-3008-6901
Jingfan ZhouShanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Peixian DongShanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Maggie Z X XiaoFaculty of Medicine, University of Alberta, Edmonton, Alberta, Canada.ORCID 0000-0002-7534-6079
Chunxia WangDepartment of Critical Care Medicine, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Yucai ZhangDepartment of Critical Care Medicine, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Joyce FuDepartment of Statistics, University of California, Riverside, Riverside, California, United States of America.ORCID 0000-0001-9202-3661
Fanxiu ZhuDepartment of Biological Science, Florida State University, Tallahassee, Flordia, United States of America.ORCID 0000-0002-4189-7008
Qiming LiangResearch Center of Translational Medicine, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.ORCID 0000-0003-4792-1547
Shanghai Jiao Tong University · CNFlorida State University · USUniversity of Alberta · CAUniversity of California, Riverside · US

Funding

Role of KSHV ORF45 in Viral Immune Evasion &ReplicationR01DE016680 · NIDCR · UNIVERSITY OF PENNSYLVANIA · PI ZHU, FANXIU · 2005 to 2015
$2.5M
Subversion of cGAS DNA sensing by KSHV: Roles of ORF52/KicGASR01DE026101 · NIDCR · FLORIDA STATE UNIVERSITY · PI ZHU, FANXIU · 2016 to 2020
$1.8M
NIDCR NIH HHS R01 DE016680NIDCR NIH HHS R01 DE026101
6 · The paper itself

Abstract

RSK1, a downstream kinase of the MAPK pathway, has been shown to regulate multiple cellular processes and is essential for lytic replication of a variety of viruses, including Kaposi's sarcoma-associated herpesvirus (KSHV). Besides phosphorylation, it is not known whether other post-translational modifications play an important role in regulating RSK1 function. We demonstrate that RSK1 undergoes robust SUMOylation during KSHV lytic replication at lysine residues K110, K335, and K421. SUMO modification does not alter RSK1 activation and kinase activity upon KSHV ORF45 co-expression, but affects RSK1 downstream substrate phosphorylation. Compared to wild-type RSK1, the overall phosphorylation level of RxRxxS*/T* motif is significantly declined in RSK1K110/335/421R expressing cells. Specifically, SUMOylation deficient RSK1 cannot efficiently phosphorylate eIF4B. Sequence analysis showed that eIF4B has one SUMO-interacting motif (SIM) between the amino acid position 166 and 170 (166IRVDV170), which mediates the association between eIF4B and RSK1 through SUMO-SIM interaction. These results indicate that SUMOylation regulates the phosphorylation of RSK1 downstream substrates, which is required for efficient KSHV lytic replication.

Indexed as

Cell LineHerpesvirus 8, HumanHost-Pathogen InteractionsHumansRibosomal Protein S6 Kinases, 90-kDaSumoylationVirus ReplicationRibosomal Protein S6 Kinases, 90-kDaRPS6KA1 protein, human

Identifiers

PMID34871326
PMCPMC8675914
OpenAlexW4200017820

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.