Evidence map›Paper›PMID 34870810›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Analysis of the Localization of NLRs upon Shigella flexneri Infection Exemplified by NOD1.

Christine Arnold, Kornelia Ellwanger, Thomas A Kufer

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 74% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Christine ArnoldDepartment of Immunology, Institute of Nutritional Medicine, University of Hohenheim, Stuttgart, Germany.
Kornelia EllwangerDepartment of Immunology, Institute of Nutritional Medicine, University of Hohenheim, Stuttgart, Germany.
Thomas A KuferDepartment of Immunology, Institute of Nutritional Medicine, University of Hohenheim, Stuttgart, Germany. thomas.kufer@uni-hohenheim.de.
University of Hohenheim · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

NOD-like receptors (NLRs) are a family of pattern recognition receptors, able to respond to conserved microbial structures and endogenous danger signals. The NLR NOD1 responds to bacterial peptidoglycan, leading to recruitment of RIPK2, following activation of NFκB and MAPK pathways. In this chapter, we describe a fluorescent light microscopic approach to analyze the subcellular distribution of NOD1 upon infection with the invasive, Gram-negative bacterial pathogen Shigella flexneri. This method is based on exogenously expressed EGFP-tagged NOD1 and describes a protocol to obtain inducible cell lines with functional NOD1 signaling. The described protocol is useful to study NOD1 function, also in living cells, using live cell imaging and can be adopted for the study of other NLR proteins.

Indexed as

Signal TransductionNF-kappa BNLR ProteinsNod1 Signaling Adaptor ProteinShigella flexneriNF-kappa BNLR ProteinsNod1 Signaling Adaptor ProteinFluorescence microscopyImmunofluorescenceLive cell imagingNOD-like receptors (NLRs)Nucleotide-binding and oligomerization domain-containing protein 1 (NOD1)Shigella flexneri infectionStable inducible cell lineTransfection of epithelial cells

Identifiers

PMID34870810
OpenAlexW4200148297

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.