Evidence map›Paper›PMID 34870749›Full record

ArticleArchives of microbiology2021

Simultaneous isolation and enumeration of virulent Vibrio cholerae and Vibrio vulnificus using an advanced MPN-PCR method.

Jae-Hwa Lee, Seul-Ki Park, Fazlurrahman Khan, Du-Min Jo, Do-Ha Lee, Min-Gyun Kang, Young-Mog Kim

Abstract read
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In one paragraph

Article in Archives of microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Microorganisms · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jae-Hwa LeeDepartment of Food Science and Technology, Pukyong National University, Busan, 48513, South Korea.
Seul-Ki ParkDepartment of Chemical and Biological Engineering, College of Engineering, University of Saskatchewan, 57 Campus Dr, Saskatoon, SK, S7N 5A9, Canada.
Fazlurrahman KhanResearch Center for Marine Integrated Bionics Technology, Pukyong National University, Busan, 48513, South Korea.
Du-Min JoDepartment of Food Science and Technology, Pukyong National University, Busan, 48513, South Korea.
Do-Ha LeeDepartment of Food Science and Technology, Pukyong National University, Busan, 48513, South Korea.
Min-Gyun KangDepartment of Food Science and Technology, Pukyong National University, Busan, 48513, South Korea.
Young-Mog KimDepartment of Food Science and Technology, Pukyong National University, Busan, 48513, South Korea. ymkim@pknu.ac.kr.ORCID http://orcid.org/0000-0002-2465-8013

Funding

Ministry of Education 2021R1A6A1A03039211
6 · The paper itself

Abstract

Vibrio cholerae and Vibrio vulnificus are critical foodborne pathogens that need to be intensively controlled for their infection due to the intake and distribution of seafood, especially raw oysters. For this reason, various methods have already been developed for the detection and enumeration of these bacteria. The most probable number (MPN)-PCR (polymerase chain reaction) method is commonly used with the selective-differential medium for the efficiency and convenience of cell enumeration. One of the most frequently used for detecting Vibrio spp. is thiosulfate-citrate-bile salts-sucrose (TCBS) agar. But this selective-differential medium can fail to distinguish between V. cholerae, V. vulnificus, and Vibrio alginolyticus. For this reason, the conventional MPN-PCR method with TCBS medium for the detection of Vibrio spp. has a problem with processing PCR two times. This study suggests a simple and minimized detection method using one-time PCR and non-NaCl Luria-Bertani (LB-0) medium culture. This detection method is based on the difference in salt requirement between V. cholerae and V. vulnificus. Employing the developed methodology, the simultaneous cell enumeration of V. cholerae and V. vulnificus can be possible at a low cost. Furthermore, this study proposes a new specific primer to detect virulence-related genes from V. cholerae and V. vulnificus. This advanced MPN-PCR method was verified using bioaccumulated pacific oysters (Crassostrea gigas) by V. cholerae and V. vulnificus.

Indexed as

OstreidaeVibrio choleraeVibrio parahaemolyticusVibrio vulnificusAnimalsPolymerase Chain ReactionEnumerationIsolationLow costMPN-PCRVirulent Vibrio spp.

Identifiers

PMID34870749

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.