ArticleeLife2021
Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Efficient Endogenous Tagging in the Sea Urchin,bioRxiv : the preprint server for biology · 2026Article
- Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide.Frontiers in zoology · 2025Review
- Cloning-Free Targeting of Endogenous Loci to Generate Fluorescent Reporters in Medaka.Bio-protocol · 2025Article
- Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata.EvoDevo · 2025Article
- Article
- Optimizing reproductive performance in pangasius catfish broodstock: A review of dietary and molecular strategies.Veterinary and animal science · 2024Review
- Modular control of vertebrate axis segmentation in time and space.The EMBO journal · 2024Article
- Tagging theZebrafish · 2024Article
- Genome editing in East African cichlids and tilapias: state-of-the-art and future directions.Open biology · 2023Review
- Review
- Fast, precise and cloning-free knock-in of reporter sequences in vivo with high efficiency.Development (Cambridge, England) · 2023Article
- Article
- Efficient knock-in method enabling lineage tracing in zebrafish.Life science alliance · 2023Article
- Article
- Genetic manipulation of betta fish.bioRxiv : the preprint server for biology · 2023Article
- CRISPR/Cas9 knockin methodology for the sea urchin embryo.Molecular reproduction and development · 2023Article
- Polarized transport of membrane and secreted proteins during lumen morphogenesis.Seminars in cell & developmental biology · 2023Review
- East African cichlid fishes.EvoDevo · 2023Review
- Endogenous tagging of multiple cellular components in the sea anemoneProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Genetic manipulation of betta fish.Frontiers in genome editing · 2023Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR/Cas9 system has been used to generate fluorescently labelled fusion proteins by homology-directed repair in a variety of species. Despite its revolutionary success, there remains an urgent need for increased simplicity and efficiency of genome editing in research organisms. Here, we establish a simplified, highly efficient, and precise strategy for CRISPR/Cas9-mediated endogenous protein tagging in medaka (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.