Evidence map›Paper›PMID 34869922›Full record

ArticleBiochemistry and biophysics reports2021

A rapid increase in lysophospholipids after geranylgeranoic acid treatment in human hepatoma-derived HuH-7 cells revealed by metabolomics analysis.

Yoshihiro Shidoji, Chieko Iwao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry and biophysics reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Yoshihiro ShidojiMolecular and Cellular Biology, Graduate School of Human Health Science, University of Nagasaki, Nagayo, Nagasaki, 851-2195, Japan.
Chieko IwaoMolecular and Cellular Biology, Graduate School of Human Health Science, University of Nagasaki, Nagayo, Nagasaki, 851-2195, Japan.
University of Nagasaki · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Geranylgeranoic acid (GGA) was developed as a preventative agent against second primary hepatoma, and was reported to induce cell death in human hepatoma cells via Toll-like receptor 4 (TLR4)-mediated pyroptosis. We recently reported that GGA is enzymatically biosynthesized from mevalonic acid in human hepatoma-derived HuH-7 cells and that endogenous GGA is found in most rat organs including the liver. An unbiased metabolomics analysis of ice-cold 50% acetonitrile extracts from control and GGA-treated cells was performed in this study to characterize the intracellular metabolic changes in GGA-induced pyroptosis and to analyze their relationship with the mechanism of GGA-induced cell death. The total positive ion chromatograms of the cellular extracts in ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry were apparently unchanged after GGA treatment, but an orthogonal partial least squares-discriminant analysis score plot clearly discriminated the intracellular metabolite profiles of GGA-treated cells from that of control cells. S-plot analysis revealed 15 potential biomarkers up-regulated by 24-h GGA treatment according to their variable importance in the projection value of more than 1, and the subsequent metabolomics analysis identified nine of these metabolites as a group of lysophospholipids containing lysophosphatidylcholine with C16:0, C20:4, or C20:3 fatty acids. The possible roles of these lysophospholipids in GGA-induced pyroptosis are discussed.

Indexed as

ATRA, all-trans retinoic acidCell deathD-MEM, Dulbecco’s modified Eagle’s mediumENPP2, ectonucleotide pyrophosphatase/phosphodiesterase 2FBS, fetal bovine serumGeranylgeranoic acidGGA, geranylgeranoic acidGSDMD, gasdermin DHepatomaHMDB, Human Metabolome DatabaseKEGG, Kyoto Encyclopedia of Genes and GenomesLCAT, lecithin cholesterol acyltransferaseLIPC, lipase CLPA, lysophosphatidic acidLPCAT, LPC acyltransferaseLPC, lysophosphatidylcholineLPE, lysophosphatidylethanolamineLPL, lysophospholipidLysophospholipidsMetabolomicsOPLS-DA, orthogonal partial least squares-discriminant analysisPCA, principal component analysisPLA2, phospholipase A2Q-Tof/MS, quadrupole time-of-flight type mass spectrometrySPH, second primary hepatomaTLR4, toll-like receptor-4UPLC, ultra-performance liquid chromatographyUPRER, unfolded protein response or endoplasmic reticulum stress responseVIP, variable importance in the projection

Identifiers

PMID34869922
PMCPMC8626837
OpenAlexW3215020111

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.