Evidence map›Paper›PMID 34869582›Full record

ArticleFrontiers in molecular biosciences2021

Stabilization of Ribosomal RNA of the Small Subunit by Spermidine in

Margarita Belinite, Iskander Khusainov, Heddy Soufari, Stefano Marzi, Pascale Romby, Marat Yusupov, Yaser Hashem

Open access · goldAbstract read
In one paragraph

Article in Frontiers in molecular biosciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Synthesis and Evaluation of Antibacterial and Antifungal ActivitiesMedicinal chemistry (Shariqah (United Arab Emirates)) · 2025
    Article
  4. Article
  5. tRNARNA (New York, N.Y.) · 2024
    Article
  6. Article
  7. Article
  8. Bio-protocol · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Margarita BeliniteInstitut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U964, CNRS UMR7104, Université de Strasbourg, Illkirch, France.
Iskander KhusainovInstitut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U964, CNRS UMR7104, Université de Strasbourg, Illkirch, France.
Heddy SoufariInstitut Européen de Chimie et Biologie (IECB), ARNA U1212, Université de Bordeaux, Pessac, France.
Stefano MarziArchitecture et Réactivité de l'ARN, CNRS 9002, Université de Strasbourg, Strasbourg, France.
Pascale RombyArchitecture et Réactivité de l'ARN, CNRS 9002, Université de Strasbourg, Strasbourg, France.
Marat YusupovInstitut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U964, CNRS UMR7104, Université de Strasbourg, Illkirch, France.
Yaser HashemArchitecture et Réactivité de l'ARN, CNRS 9002, Université de Strasbourg, Strasbourg, France.
Centre National de la Recherche Scientifique · FRUniversité de Strasbourg · FRInstitut de Biologie Moléculaire et Cellulaire · FRUniversité de Bordeaux · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cryo-electron microscopy is now used as a method of choice in structural biology for studying protein synthesis, a process mediated by the ribosome machinery. In order to achieve high-resolution structures using this approach, one needs to obtain homogeneous and stable samples, which requires optimization of ribosome purification in a species-dependent manner. This is especially critical for the bacterial small ribosomal subunit that tends to be unstable in the absence of ligands. Here, we report a protocol for purification of stable 30 S from the Gram-positive bacterium

Indexed as

ribosomal RNAribosome 70 SRNA stabilityStaphylococcus aureustranslation

Identifiers

PMID34869582
PMCPMC8637172
OpenAlexW3217419154

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.