ArticlePlant physiology2022
Auxin biosynthesis maintains embryo identity and growth during BABY BOOM-induced somatic embryogenesis.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 70 citations in OpenAlex.
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- From pollen precursor to totipotent embryo: a model system for plant cell developmental plasticity.Plant cell reports · 2026Review
- HcBBM promotes callus differentiation and shoot regeneration by coordinating auxin signaling in Hemsleya chinensis.BMC plant biology · 2026Article
- MONOPTEROS isoform MP11ir plays a role during somatic embryogenesis in Arabidopsis thaliana.Plant physiology · 2025Article
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- Mechanism and Application of Developmental Factors in Plant Genetic Transformation.International journal of molecular sciences · 2025Review
- The triggering mechanism for predominant hormonal signal production in fleshy fruit ripening.Molecular horticulture · 2025Review
- Transcriptional Dynamics Underlying Somatic Embryogenesis inPlants (Basel, Switzerland) · 2025Article
- Strigolactone insensitivity affects the hormonal homeostasis in barley.Scientific reports · 2025Article
- Electrochemical impedance spectroscopy as a micropropagation monitoring tool for plants: A case study of tamarilloiScience · 2025Article
- Transcriptomic and metabolomic analysis clarify the molecular mechanisms underlying the formation of sexual and apomictic Persian walnut (Frontiers in plant science · 2025Article
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- How does light regulate plant regeneration?Frontiers in plant science · 2024Review
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- Epigenetic modifications and miRNAs determine the transition of somatic cells into somatic embryos.Plant cell reports · 2023Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Somatic embryogenesis is a type of plant cell totipotency where embryos develop from nonreproductive (vegetative) cells without fertilization. Somatic embryogenesis can be induced in vitro by auxins, and by ectopic expression of embryo-expressed transcription factors like the BABY BOOM (BBM) AINTEGUMENTA-LIKE APETALA2/ETHYLENE RESPONSE FACTOR domain protein. These different pathways are thought to converge to promote auxin response and biosynthesis, but the specific roles of the endogenous auxin pathway in somatic embryogenesis induction have not been well-characterized. Here we show that BBM transcriptionally regulates the YUCCA3 (YUC3) and YUC8 auxin biosynthesis genes during BBM-mediated somatic embryogenesis in Arabidopsis (Arabidopsis thaliana) seedlings. BBM induced local and ectopic YUC3 and YUC8 expression in seedlings, which coincided with increased DR5 auxin response and indole-3-acetic acid (IAA) biosynthesis and with ectopic expression of the WOX2 embryo reporter. YUC-driven auxin biosynthesis was required for BBM-mediated somatic embryogenesis, as the number of embryogenic explants was reduced by ca. 50% in yuc3 yuc8 mutants and abolished after chemical inhibition of YUC enzyme activity. However, a detailed YUC inhibitor time-course study revealed that YUC-dependent IAA biosynthesis is not required for the re-initiation of totipotent cell identity in seedlings. Rather, YUC enzymes are required later in somatic embryo development for the maintenance of embryo identity and growth. This study resolves a long-standing question about the role of endogenous auxin biosynthesis in transcription factor-mediated somatic embryogenesis and also provides an experimental framework for understanding the role of endogenous auxin biosynthesis in other in planta and in vitro embryogenesis systems.
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