ArticleJournal of neuroscience methods2022
Dynamics of isoflurane-induced vasodilation and blood flow of cerebral vasculature revealed by multi-exposure speckle imaging.
Article in Journal of neuroscience methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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57 citing papers in PubMed, 84 citations in OpenAlex.
- MIMAS: An open-source microlens-array miniature microscope for accessible large-field cortical imaging in freely behaving mice.Science advances · 2026Article
- Microbubble Track-Based Functional Ultrasound Localization Microscopy in Awake Mice.IEEE transactions on medical imaging · 2026Article
- Brief, Repeated Isoflurane Anaesthesia Elicits Sex-Dependent Behavioural, Cerebrovascular and Microglial Alterations in Mice.The European journal of neuroscience · 2026Article
- Ultrasound localisation microscopy tracks testicular microvascular adaptations to endocrine function in male infertility.EBioMedicine · 2026Article
- Noninvasive whole-brain imaging of glymphatic dynamics.Science advances · 2026Article
- Endothelial GPR68 Is Essential for Arteriogenesis and Represents a Therapeutic Target in a Model of Peripheral Artery Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Imaging of Neurovascular Activity during Motor Responses Evoked by Displacement-guided Focused Ultrasound (DgFUS) Neuromodulation in Mice.Research square · 2026Article
- Fetal Tadalafil Exposure Increases Pulmonary Blood Flow and Reduces Right to Left Heart Shunting through the Foramen Ovale.Neonatology · 2026Article
- Retinal OCT Parameters and Physiological Dynamics as an Effect of Anesthesia in C57BL/6J Mice.Investigative ophthalmology & visual science · 2026Article
- Early identification of umbilical blood flow restriction and maternal placental hypoperfusion with photoacoustic imaging.Photoacoustics · 2026Article
- Effect of hypoglycemia on the diameter of cerebral vasculature in vivo and cerebral ischemia-reperfusion injury in mice.Experimental neurology · 2026Article
- Imaging vascular characteristics and glycolytic metabolism of glioblastoma in a chick embryo model usingMolecular imaging and biology · 2026Article
- Persistent dysmorphology and dysfunction of the brain microvasculature following repeated trauma.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026Article
- Perivascular spaces balance vascular dimensions without impairing brain clearance mechanisms.Fluids and barriers of the CNS · 2026Article
- Blood flow analysis of retinal neovascularisations in a VLDLR mouse model using contrast-enhanced optical coherence tomography.Biomedical optics express · 2026Article
- Cortical OProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Assessing cerebral capillary function and stalling using single capillary reporters in ultrasound localization microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cardiac pulse transfer along the cerebral microvascular network captured by laser speckle contrast imaging.Biomedical optics express · 2026Article
- Cortical Microvascular Pulsatility in the Aging Mouse Brain and the Confounding Effects of Anesthesia.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
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5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAnesthetized animal models are used extensively during neurophysiological and behavioral studies despite systemic effects from anesthesia that undermine both accurate interpretation and translation to awake human physiology. The majority of work examining the impact of anesthesia on cerebral blood flow (CBF) has been restricted to before and after measurements with limited spatial resolution. NEW
methodWe used multi-exposure speckle imaging (MESI), an advanced form of laser speckle contrast imaging (LSCI), to characterize the dynamics of isoflurane anesthesia induction on cerebral vasculature and blood flow in the mouse brain.
resultsThe large anatomical changes caused by isoflurane are depicted with wide-field imagery and video highlighting the induction of general anesthesia. Within minutes of exposure, both vessel diameter and blood flow increased drastically compared to the awake state and remained elevated for the duration of imaging. An examination of the dynamics of anesthesia induction reveals that blood flow increased faster in arteries than in veins or parenchyma regions. COMPARISON WITH EXISTING
methodsMESI offers robust hemodynamic measurements across large fields-of-view and high temporal resolutions sufficient for continuous visualization of cerebrovascular events featuring major changes in blood flow.
conclusionThe large alterations caused by isoflurane anesthesia to the cortical vasculature and CBF are readily characterized using MESI. These changes are unrepresentative of normal physiology and provide further evidence that neuroscience experiments would benefit from transitioning to un-anesthetized awake animal models.
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