Evidence map›Paper›PMID 34863780›Full record

ArticleJournal of molecular biology2022

Real-Time Single-Molecule Studies of RNA Polymerase-Promoter Open Complex Formation Reveal Substantial Heterogeneity Along the Promoter-Opening Pathway.

Anssi M Malinen, Jacob Bakermans, Emil Aalto-Setälä, Martin Blessing, David L V Bauer, Olena Parilova, Georgiy A Belogurov, David Dulin, Achillefs N Kapanidis

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of molecular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Anssi M MalinenDepartment of Life Technologies, University of Turku, 20014 Turku, Finland; Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, UK. Electronic address: anssi.malinen@utu.fi.
Jacob BakermansBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, UK.
Emil Aalto-SetäläDepartment of Life Technologies, University of Turku, 20014 Turku, Finland.
Martin BlessingBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, UK; Max Planck Institute for the Science of Light, Staudtstraße 2, 91058 Erlangen, Germany.
David L V BauerBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, UK; RNA Virus Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
Olena ParilovaDepartment of Life Technologies, University of Turku, 20014 Turku, Finland.
Georgiy A BelogurovDepartment of Life Technologies, University of Turku, 20014 Turku, Finland.
David DulinBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, UK; Junior Research Group 2, Interdisciplinary Center for Clinical Research, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Cauerstr. 3, 91058 Erlangen, Germany; Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, the Netherlands.
Achillefs N KapanidisBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, UK; Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford. Electronic address: achillefs.kapanidis@physics.ox.ac.uk.

Funding

Biotechnology and Biological Sciences Research Council BB/H01795X/1Cancer Research UK FC011104Medical Research Council FC011104Wellcome Trust 110164/Z/15/ZWellcome Trust FC011104
6 · The paper itself

Abstract

The expression of most bacterial genes commences with the binding of RNA polymerase (RNAP)-σ

Indexed as

Promoter Regions, GeneticCryoelectron MicroscopyDNA, BacterialDNA-Directed RNA PolymerasesEscherichia coliGenes, BacterialHoloenzymesModels, MolecularNucleic Acid ConformationProtein BindingProtein ConformationTranscription, GeneticTranscription Initiation, GeneticDNA, BacterialDNA-Directed RNA PolymerasesHoloenzymesmolecular mechanismreaction pathwaytotal internal reflection fluorescence microscopytranscription initiationσ factor

Identifiers

PMID34863780
PMCPMC8783055

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.