ArticleBMC plant biology2021
A cotransformation system of the unicellular red alga Cyanidioschyzon merolae with blasticidin S deaminase and chloramphenicol acetyltransferase selectable markers.
Article in BMC plant biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Sexual life cycle establishes the unicellular red algae Cyanidiophyceae as a genetically tractable model for eukaryotic evolution.The Plant cell · 2026Article
- Fascinating single-cell red algae: models for evolution and adaptation.The New phytologist · 2026Review
- Genome Position Does Not Impact Transgene Expression Efficiency in the Ancient Red AlgabioRxiv : the preprint server for biology · 2026Article
- IPTG- and estradiol-inducible gene expression systems in the unicellular red alga Cyanidioschyzon merolae.Plant physiology · 2026Article
- Costs of photosynthesis and cellular remodeling in trophic transitions of the unicellular red alga Galdieria partita.Communications biology · 2025Article
- Development of a rapamycin-inducible protein-knockdown system in the unicellular red alga Cyanidioschyzon merolae.Plant physiology · 2024Article
- Engineered ketocarotenoid biosynthesis in the polyextremophilic red microalgaMetabolic engineering communications · 2023Article
- How Light Modulates the Growth ofCells · 2023Article
- Cultivation of the polyextremophileFrontiers in microbiology · 2023Article
- Life cycle and functional genomics of the unicellular red algaProceedings of the National Academy of Sciences of the United States of America · 2022Article
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3 authors.
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Abstract
backgroundThe unicellular red alga Cyanidioschyzon merolae exhibits a very simple cellular and genomic architecture. In addition, procedures for genetic modifications, such as gene targeting by homologous recombination and inducible/repressible gene expression, have been developed. However, only two markers for selecting transformants, uracil synthase (URA) and chloramphenicol acetyltransferase (CAT), are available in this alga. Therefore, manipulation of two or more different chromosomal loci in the same strain in C. merolae is limited.
resultsThis study developed a nuclear targeting and transformant selection system using an antibiotics blasticidin S (BS) and the BS deaminase (BSD) selectable marker by homologous recombination in C. merolae. In addition, this study has succeeded in simultaneously modifying two different chromosomal loci by a single-step cotransformation based on the combination of BSD and CAT selectable markers. A C. merolae strain that expresses mitochondrion-targeted mSCARLET (with the BSD marker) and mVENUS (with the CAT marker) from different chromosomal loci was generated with this procedure.
conclusionsThe newly developed BSD selectable marker enables an additional genetic modification to the already generated C. merolae transformants based on the URA or CAT system. Furthermore, the cotransformation system facilitates multiple genetic modifications. These methods and the simple nature of the C. merolae cellular and genomic architecture will facilitate studies on several phenomena common to photosynthetic eukaryotes.
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