Evidence map›Paper›PMID 34862427›Full record

ArticleScientific reports2021

Myeloid-associated differentiation marker is a novel SP-A-associated transmembrane protein whose expression on airway epithelial cells correlates with asthma severity.

Alane Blythe C Dy, Paul R Langlais, Natalie K Barker, Kenneth J Addison, Sasipa Tanyaratsrisakul, Scott Boitano, Stephanie A Christenson, Monica Kraft, Deborah Meyers, Eugene R Bleecker and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 8 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Alane Blythe C DyClinical Translational Sciences, University of Arizona Health Sciences, Tucson, AZ, 85721, USA.
Paul R LanglaisDivision of Endocrinology, Department of Medicine, University of Arizona, Tucson, AZ, 85724, USA.
Natalie K BarkerDivision of Endocrinology, Department of Medicine, University of Arizona, Tucson, AZ, 85724, USA.
Kenneth J AddisonAsthma and Airway Disease Research Center, University of Arizona, Tucson, AZ, 85724, USA.
Sasipa TanyaratsrisakulAsthma and Airway Disease Research Center, University of Arizona, Tucson, AZ, 85724, USA.
Scott BoitanoAsthma and Airway Disease Research Center, University of Arizona, Tucson, AZ, 85724, USA.
Stephanie A ChristensonDivision of Pulmonary, Critical Care, Allergy and Sleep Medicine, Department of Medicine, University of California San Francisco, San Francisco, CA, 94117, USA.
Monica KraftAsthma and Airway Disease Research Center, University of Arizona, Tucson, AZ, 85724, USA.
Deborah MeyersAsthma and Airway Disease Research Center, University of Arizona, Tucson, AZ, 85724, USA.
Eugene R BleeckerAsthma and Airway Disease Research Center, University of Arizona, Tucson, AZ, 85724, USA.
Xingnan LiAsthma and Airway Disease Research Center, University of Arizona, Tucson, AZ, 85724, USA.
Julie G LedfordAsthma and Airway Disease Research Center, University of Arizona, Tucson, AZ, 85724, USA. jledford@email.arizona.edu.
University of Arizona · USUniversity of California System · US

Funding

Rapid saliva antigen test for SARS-CoV-2 detectionU01HL146002 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI ARONICA, MARK A, CASTRO, MARIO · 2019 to 2023
$17.3M
Tollip inhibits IL-33 signaling during airway influenza virus infectionU19AI125357 · NIAID · UNIVERSITY OF ARIZONA · PI CHU, HONG W · 2016 to 2025
$14.7M
Defining mechanisms of CC16 on epithelial-driven host responses to pathogensR01HL142769 · NHLBI · UNIVERSITY OF ARIZONA · PI Julie Gunnells Ledford · 2019 to 2026
$4.2M
Surfactant Protein-A regulation of eosinophil apoptosis in asthmaR01HL125602 · NHLBI · UNIVERSITY OF ARIZONA · PI LEDFORD, JULIE GUNNELLS · 2015 to 2019
$1.9M
NHLBI NIH HHS R01 HL125602NHLBI NIH HHS R01 HL142769NHLBI NIH HHS U01 HL146002NIAID NIH HHS U19 AI125357
6 · The paper itself

Abstract

Surfactant protein A (SP-A) is well-known for its protective role in pulmonary immunity. Previous studies from our group have shown that SP-A mediates eosinophil activities, including degranulation and apoptosis. In order to identify potential binding partners on eosinophils for SP-A, eosinophil lysates were subjected to SP-A pull-down and tandem mass spectrometry (MS/MS) analysis. We identified one membrane-bound protein, myeloid-associated differentiation marker (MYADM), as a candidate SP-A binding partner. Blocking MYADM on mouse and human eosinophils ex vivo prevented SP-A from inducing apoptosis; blocking MYADM in vivo led to increased persistence of eosinophilia and airway hyper-responsiveness in an ovalbumin (OVA) allergy model and increased airways resistance and mucus production in a house dust mite (HDM) asthma model. Examination of a subset of participants in the Severe Asthma Research Program (SARP) cohort revealed a significant association between epithelial expression of MYADM in asthma patients and parameters of airway inflammation, including: peripheral blood eosinophilia, exhaled nitric oxide (FeNO) and the number of exacerbations in the past 12 months. Taken together, our studies provide the first evidence of MYADM as a novel SP-A-associated protein that is necessary for SP-A to induce eosinophil apoptosis and we bring to light the potential importance of this previously unrecognized transmembrane protein in patients with asthma.

Indexed as

AdultAnimalsAsthmaChromatography, LiquidDisease Models, AnimalEosinophilsFemaleHumansMaleMiceMiddle AgedMyelin and Lymphocyte-Associated Proteolipid ProteinsPatient AcuityPulmonary Surfactant-Associated Protein APyroglyphidaeTandem Mass SpectrometryMYADM protein, humanMyadm protein, mouseMyelin and Lymphocyte-Associated Proteolipid ProteinsPulmonary Surfactant-Associated Protein A

Identifiers

PMID34862427
PMCPMC8642528
OpenAlexW3214794808

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.