ArticleNature communications2021
CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Female- or male-biased offspring generated by targeted distortion of sex chromosome transmission in the mouse.Genetics · 2026Article
- Y-linked editors for invasive rodent control.Molecular biology and evolution · 2026Article
- CRISPR/Cas9-mediated TOP1 knockout in chicken DF-1 cells reveals its critical role in apoptosis regulation and genomic stability.Journal of animal science · 2026Article
- CRISPR-Cas9 in the Tailoring of Genetically Engineered Animals.Current issues in molecular biology · 2025Review
- Maximising Eradication Potential of Rat Gene Drives Using a Two-Target Homing Rescue Strategy: Spatial Modelling of Empirical Data.Molecular ecology · 2025Article
- Systematic identification of Y-chromosome gene functions in mouse spermatogenesis.Science (New York, N.Y.) · 2025Article
- Advances in immunological sorting of X and Y chromosome-bearing sperm: from proteome to sex-specific proteins.Frontiers in veterinary science · 2025Review
- Identification of Site in theGenes · 2024Article
- Gender Control of Mouse Embryos by Activation of TLR7/8 on X Sperm via Ligands dsRNA-40 and dsRNA-DR.Molecules (Basel, Switzerland) · 2024Article
- Unmasking the Adverse Impacts of Sex Bias on Science and Research Animal Welfare.Animals : an open access journal from MDPI · 2023Review
- The History and Prospects of Rabbit Sperm Sexing.Veterinary sciences · 2023Review
- Knockout ofAnimals : an open access journal from MDPI · 2023Article
- Article
- Rapid, visual and highly sensitive sexing of bovine embryos by recombinase polymerase amplification with CFI staining.Heliyon · 2023Article
- Governance of Heritable Human Gene Editing World-Wide and Beyond.International journal of environmental research and public health · 2022Article
- Sex differences in the intergenerational inheritance of metabolic traits.Nature metabolism · 2022Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Animals are essential genetic tools in scientific research and global resources in agriculture. In both arenas, a single sex is often required in surplus. The ethical and financial burden of producing and culling animals of the undesired sex is considerable. Using the mouse as a model, we develop a synthetic lethal, bicomponent CRISPR-Cas9 strategy that produces male- or female-only litters with one hundred percent efficiency. Strikingly, we observe a degree of litter size compensation relative to control matings, indicating that our system has the potential to increase the yield of the desired sex in comparison to standard breeding designs. The bicomponent system can also be repurposed to generate postnatal sex-specific phenotypes. Our approach, harnessing the technological applications of CRISPR-Cas9, may be applicable to other vertebrate species, and provides strides towards ethical improvements for laboratory research and agriculture.
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Registered trials
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