Evidence map›Paper›PMID 34857757›Full record

ArticleNature communications2021

Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors.

Wen Hao Neo, Yiran Meng, Alba Rodriguez-Meira, Muhammad Z H Fadlullah, Christopher A G Booth, Emanuele Azzoni, Supat Thongjuea, Marella F T R de Bruijn, Sten Eirik W Jacobsen, Adam J Mead and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 3 countries.

Wen Hao NeoHaematopoietic Stem Cell Biology Laboratory, MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, OX3 9DS, UK. wenhao.neo@cruk.manchester.ac.uk.ORCID http://orcid.org/0000-0002-6827-3027
Yiran MengMRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, OX3 9DS, UK.ORCID http://orcid.org/0000-0002-9333-2383
Alba Rodriguez-MeiraHaematopoietic Stem Cell Biology Laboratory, MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, OX3 9DS, UK.
Muhammad Z H FadlullahStem Cell Biology Group, Cancer Research UK Manchester Institute, The University of Manchester, Macclesfield, SK10 4TG, UK.
Christopher A G BoothHaematopoietic Stem Cell Biology Laboratory, MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, OX3 9DS, UK.ORCID http://orcid.org/0000-0003-3841-6637
Emanuele AzzoniMRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, OX3 9DS, UK.ORCID http://orcid.org/0000-0002-4572-5692
Supat ThongjueaMRC WIMM Centre for Computational Biology, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, OX3 9DS, UK.ORCID http://orcid.org/0000-0002-9129-4694
Marella F T R de BruijnMRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, OX3 9DS, UK.ORCID http://orcid.org/0000-0002-4934-4125
Sten Eirik W JacobsenHaematopoietic Stem Cell Biology Laboratory, MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, OX3 9DS, UK.
Adam J MeadHaematopoietic Stem Cell Biology Laboratory, MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, OX3 9DS, UK. adam.mead@imm.ox.ac.uk.ORCID http://orcid.org/0000-0001-8522-1002
Georges LacaudStem Cell Biology Group, Cancer Research UK Manchester Institute, The University of Manchester, Macclesfield, SK10 4TG, UK. georges.lacaud@cruk.manchester.ac.uk.ORCID http://orcid.org/0000-0002-5630-2417
University of Oxford · GBUniversity of Manchester · GBKarolinska University Hospital · SE

Funding

Cancer Research UK 26988Cancer Research UK 27125Cancer Research UK 27723Cancer Research UK C42639/A26988Cancer Research UK C5759/A27412Medical Research Council G0501838Medical Research Council G0801073Medical Research Council G0902418Medical Research Council G84/6443Medical Research Council MC_UU_00016/15Medical Research Council MC_UU_00016/2Medical Research Council MC_UU_00016/5Medical Research Council MC_UU_12009/16Medical Research Council MC_UU_12009/2Medical Research Council MC_UU_12009/5Medical Research Council MC_UU_12025Medical Research Council MR/L006340/1
6 · The paper itself

Abstract

Yolk sac (YS) hematopoiesis is critical for the survival of the embryo and a major source of tissue-resident macrophages that persist into adulthood. Yet, the transcriptional and epigenetic regulation of YS hematopoiesis remains poorly characterized. Here we report that the epigenetic regulator Ezh2 is essential for YS hematopoiesis but dispensable for subsequent aorta-gonad-mesonephros (AGM) blood development. Loss of EZH2 activity in hemogenic endothelium (HE) leads to the generation of phenotypically intact but functionally deficient erythro-myeloid progenitors (EMPs), while the generation of primitive erythroid cells is not affected. EZH2 activity is critical for the generation of functional EMPs at the onset of the endothelial-to-hematopoietic transition but subsequently dispensable. We identify a lack of Wnt signaling downregulation as the primary reason for the production of non-functional EMPs. Together, our findings demonstrate a critical and stage-specific role of Ezh2 in modulating Wnt signaling during the generation of EMPs from YS HE.

Indexed as

Gene Expression Regulation, DevelopmentalAnimalsCell DifferentiationEmbryo, MammalianEnhancer of Zeste Homolog 2 ProteinEpigenesis, GeneticErythroid CellsFemaleFetusGenes, ReporterHematopoiesisLiverLuminescent ProteinsMaleMiceMice, Inbred C57BLEnhancer of Zeste Homolog 2 ProteinEzh2 protein, mouseLuminescent ProteinsNpc2 protein, mouseRed Fluorescent ProteinVesicular Transport Proteins

Identifiers

PMID34857757
PMCPMC8640066
OpenAlexW3215535560

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.