ArticleNature communications2021
Catalytic flexibility of rice glycosyltransferase OsUGT91C1 for the production of palatable steviol glycosides.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 61 citations in OpenAlex.
- Development of In Vitro and In Planta Strategies for Efficient Production of High-Value Steviol Glycosides.Plant biotechnology journal · 2026Article
- Discovery and engineering of UDP-glycosyltransferase and its applications in natural sweetener glycosylation.World journal of microbiology & biotechnology · 2026Review
- A Comprehensive Review on Steviol Glycosides: Sources, Properties, Bioactivities, Sensory-Functional Enhancement and Bioproduction Strategies.Plants (Basel, Switzerland) · 2026Review
- The progress on stevia (Frontiers in nutrition · 2026Review
- Structural insights into the catalytic mechanism of the phenylethanoid glycoside rhamnosyltransferase UGT79G15 from Rehmannia glutinosa.Plant communications · 2025Article
- Integrated genomic and DNA methylome analyses reveal epigenetic regulation of stevia glycoside biosynthesis inHorticulture research · 2025Article
- A Near Telomere-To-Telomere Genome Assembly of Coffea arabica (Mundo Novo) Provides Insights Into Its Secondary Metabolism.Molecular ecology resources · 2025Article
- Characterization ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Molecular characterization and structural basis of a promiscuous glycosyltransferase for β-(1,6) oligoglucoside chain glycosides biosynthesis.Plant biotechnology journal · 2025Article
- Characterization of a 4'-O-rhamnosyltransferase and de novo biosynthesis of bioactive steroidal triglycosides from Paris polyphylla.Plant communications · 2025Article
- Biosynthesis and metabolic engineering of natural sweeteners.AMB Express · 2025Review
- Structural Insights into the Substrate Recognition of Ginsenoside Glycosyltransferase Pq3-O-UGT2.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Enhanced Production of Rebaudioside D and Rebaudioside M through V155T Substitution in the Glycosyltransferase UGT91D2 fromJournal of agricultural and food chemistry · 2025Article
- An efficient C-glycoside production platform enabled by rationally tuning the chemoselectivity of glycosyltransferases.Nature communications · 2024Article
- Identification of a 301 bp promoter core region of the SrUGT91D2 gene from Stevia rebaudiana that contributes to hormone and abiotic stress inducibility.BMC plant biology · 2024Article
- Advances in the Structures, Pharmacological Activities, and Biosynthesis of Plant Diterpenoids.Journal of microbiology and biotechnology · 2024Review
- Functional characterization, structural basis, and protein engineering of a rare flavonoid 2'-Acta pharmaceutica Sinica. B · 2024Article
- Structural insights into the catalytic selectivity of glycosyltransferase SgUGT94-289-3 towards mogrosides.Nature communications · 2024Article
- Enhancing the thermostability and activity of glycosyltransferase UGT76G1 via computational design.Communications chemistry · 2023Article
- Insights into the missing apiosylation step in flavonoid apiosides biosynthesis of Leguminosae plants.Nature communications · 2023Article
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Authors and funding
14 authors at 3 institutions in 2 countries.
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Abstract
Steviol glycosides are the intensely sweet components of extracts from Stevia rebaudiana. These molecules comprise an invariant steviol aglycone decorated with variable glycans and could widely serve as a low-calorie sweetener. However, the most desirable steviol glycosides Reb D and Reb M, devoid of unpleasant aftertaste, are naturally produced only in trace amounts due to low levels of specific β (1-2) glucosylation in Stevia. Here, we report the biochemical and structural characterization of OsUGT91C1, a glycosyltransferase from Oryza sativa, which is efficient at catalyzing β (1-2) glucosylation. The enzyme's ability to bind steviol glycoside substrate in three modes underlies its flexibility to catalyze β (1-2) glucosylation in two distinct orientations as well as β (1-6) glucosylation. Guided by the structural insights, we engineer this enzyme to enhance the desirable β (1-2) glucosylation, eliminate β (1-6) glucosylation, and obtain a promising catalyst for the industrial production of naturally rare but palatable steviol glycosides.
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