ArticleBMC research notes2021
Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils.
Article in BMC research notes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Seasonal and Regional Variation of the Chemical Composition and Antimicrobial Activity of Essential Oils From Piper marginatum Jacq.Chemistry & biodiversity · 2026Article
- Exploitation of Nanoparticle-Essential Oil Combinations to Enhance the Efficacy of Antimicrobial Agents AgainstPharmaceutics · 2026Article
- Article
- Protocol for determining minimum inhibitory concentrations of essential oils against bacterial pathogens using broth microdilution.STAR protocols · 2026Article
- Phenotypic and Genomic Characterization of Novel Straboviridae Bacteriophages Targeting Multidrug-ResistantMicroorganisms · 2026Article
- Biocatalytic structural diversification of saponins in Zygophyllum decumbens: HR-LC-ESI-TOF-MS/MS profiling and bioactivity modulation.BMC complementary medicine and therapies · 2026Article
- Antibacterial activity of essential oils from Brocchia cinerea, Artemisia campestris and Origanum vulgare growing in Algeria against antibiotic-resistant foodborne pathogens.Scientific reports · 2026Article
- Integrative In vitro and In Silico analysis of marine Actinobacterium,Toxicology research · 2025Article
- Production, characterization, and antimicrobial activity of polyhydroxyalkanoates synthesized byRSC advances · 2025Article
- Coating silicon catheters with the optimized and stable carotenoid bioproduct from Micrococcus luteus inhibited the biofilm formation by multidrug-resistant Enterococcus faecalis via downregulation of GelE gene expression.Microbial cell factories · 2025Article
- In vitro and in vivo investigation of the antibacterial, antivirulence, and antiquorum sensing activities of β-carotene against difficult-to-treat resistant Pseudomonas aeruginosa.BMC microbiology · 2025Article
- High-throughput clinical antimicrobial susceptibility testing and drug-resistant subpopulation detection in Gram-negative bacteria.Microbiology spectrum · 2025Article
- Comparison of In Vitro Methods for Assaying the Antibacterial Activity of a Mix of Natural Essential Oils Against Zoonotic Bacteria.Microorganisms · 2025Article
- Isolation and Genomic Analysis ofAntibiotics (Basel, Switzerland) · 2025Article
- Timbe (Pharmaceuticals (Basel, Switzerland) · 2025Article
- Antimicrobial activity ofPeerJ · 2025Article
- Methods for Determination of Antimicrobial Activity of Essential Oils In Vitro-A Review.Plants (Basel, Switzerland) · 2024Review
- Essential Oils from Southern Italian Aromatic Plants Synergize with Antibiotics againstAntibiotics (Basel, Switzerland) · 2024Article
- Anti-Heliyon · 2024Article
- Chemodiversity and Bioactivity of the Essential Oils ofInternational journal of molecular sciences · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
Abstract
objectiveThe aim of the present study was to adapt and optimize a broth microdilution method and compare it to the agar dilution method for the evaluation of activity of essential oils from medicinal plants against Gram-negative bacteria. Based on bibliographic research, active and not active oils were selected. The sensitivity and specificity were established as parameters for validation. The comparison between both methods was made using contingency analysis tables, based on the observed frequencies. For both methods, the minimum inhibitory concentration was determined against Escherichia coli strains, in an essential oil concentration range between 0.03 and 0.48% (v/v).
resultsA stable emulsion formation was achieved with the addition of Tween 80 and constant agitation, guaranteeing the continuous contact of oil with bacteria (critical step in the microdilution method). The statistical analysis of results obtained with both methods presented a good sensitivity and specificity (100% in both cases), which let us correctly discriminate between active and non-active oils. The values obtained for the minimal inhibitory concentration were independent of the technique used. Finally, the obtained results show that the validated microtechnique allows important diminishment of time and resources for investigations dealing with essential oils or lipophilic extracts evaluation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.