Evidence map›Paper›PMID 34855073›Full record

ArticleJournal of fluorescence2022

Effect of pH on the Conformational Transition of Silk Fibroin in Aqueous Solution Monitored by Thioflavin-T Fluorescence.

Ben Jia, Lan Jia, Jingxin Zhu

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of fluorescence, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Microcompartmentalization Controls Silk Feedstock Rheology.Langmuir : the ACS journal of surfaces and colloids · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Ben JiaCollege of Material Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, P. R. China.
Lan JiaCollege of Material Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, P. R. China. jialan@tyut.edu.cn.ORCID http://orcid.org/0000-0001-7005-5873
Jingxin ZhuCollege of Material Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, P. R. China.
Taiyuan University of Technology · CNTaiyuan University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this work, the potential application of the fluorescence dye Thioflavin-T (ThT), which can specifically bind to amyloid, as a powerful tool for monitoring secondary structural transitions of silk fibroin (SF) induced by pH in low solution concentrations was examined. Results showed that ThT emission intensities substantially increased when pH decreased from 6.8 to 4.8. This increase may be ascribed to conformational transitions from random coil to β-sheet. The morphology and secondary structure of SF were also investigated via TEM, AFM and circular dichroism spectroscopy. The information obtained herein can be utilized not only for the development of convenient and efficient noninvasive method for monitoring the assembly behavior of SF in aqueous solution but also for in vitro fluorescence imaging.

Indexed as

BenzothiazolesFluorescent DyesWaterFibroinsHydrogen-Ion ConcentrationProtein ConformationSolutionsSpectrometry, FluorescenceBenzothiazolesFibroinsFluorescent DyesSolutionsthioflavin TWaterConformational transitionFluorescence spectroscopyProtein structuresSilk fibroinThioflavin-T

Identifiers

PMID34855073
OpenAlexW3217129524

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.