ArticleMicrobial ecology2023
Intercomparison of Two Fluorescent Dyes to Visualize Parasitic Fungi (Chytridiomycota) on Phytoplankton.
Article in Microbial ecology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- True cyst formation underlies persistence and drug tolerance in Tritrichomonas foetus.Nature communications · 2026Article
- Fungal parasites infecting NNature communications · 2026Article
- Salinity and diatom host community jointly shape chytrid parasite diversity along a natural salinity gradient.ISME communications · 2026Article
- Phytoplankton Under Pressure: Temperature, Precipitation and Cyanobacterial Blooms as Drivers of Chytrid Infections.Environmental microbiology reports · 2025Article
- Needle in a Haystack: A Droplet Digital Polymerase Chain Reaction Assay to Detect Rare Helminth Parasites Infecting Natural Host Populations.Molecular ecology resources · 2025Article
- Dynamics of zoosporic parasites in summer phytoplankton communities of the Baltic Sea.FEMS microbiology ecology · 2025Article
- Chytrid fungi infecting Arctic microphytobenthic communities under varying salinity conditions.Scientific reports · 2024Article
- Metabarcoding reveals ecologically distinct fungal assemblages in river and groundwater along an Austrian alpine to lowland gradient.FEMS microbiology ecology · 2024Article
- Biodiversity of microorganisms in the Baltic Sea: the power of novel methods in the identification of marine microbes.FEMS microbiology reviews · 2024Review
- Fungal parasitism on diatoms alters formation and bio-physical properties of sinking aggregates.Communications biology · 2023Article
- Novel parasitic chytrids infecting snow algae in an alpine snow ecosystem in Japan.Frontiers in microbiology · 2023Article
- Antarctic Glacial Meltwater Impacts the Diversity of Fungal Parasites Associated With Benthic Diatoms in Shallow Coastal Zones.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Fungal microparasites (here chytrids) are widely distributed and yet, they are often overlooked in aquatic environments. To facilitate the detection of microparasites, we revisited the applicability of two fungal cell wall markers, Calcofluor White (CFW) and wheat germ agglutinin (WGA), for the direct visualization of chytrid infections on phytoplankton in laboratory-maintained isolates and field-sampled communities. Using a comprehensive set of chytrid-phytoplankton model pathosystems, we verified the staining pattern on diverse morphological structures of chytrids via fluorescence microscopy. Empty sporangia were stained most effectively, followed by encysted zoospores and im-/mature sporangia, while the staining success was more variable for rhizoids, stalks, and resting spores. In a few instances, the staining was unsuccessful (mostly with WGA), presumably due to insufficient cell fixation, gelatinous cell coatings, and multilayered cell walls. CFW and WGA staining could be done in Utermöhl chambers or on polycarbonate filters, but CFW staining on filters seemed less advisable due to high background fluorescence. To visualize chytrids, 1 µg dye mL
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