ArticlePLoS pathogens2021
VgrG-dependent effectors and chaperones modulate the assembly of the type VI secretion system.
Article in PLoS pathogens, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 48 citations in OpenAlex.
- Aeromonas dhakensis as an emerging invasive pathogen: a case series highlighting distinct carbapenem resistance and diagnostic challenge.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- An ubiquitous antibacterial toxin from human gut bacteria engenders neonatal colonization advantage.Nature communications · 2026Article
- A T4SS effector directly disarms competing antibacterial T6SSs by hijacking their conserved spike component.Science China. Life sciences · 2026Article
- Gene-in-gene coding generates dual-isoform Fha condensates to control type VI secretion system assembly.Nature communications · 2026Article
- Pan-genome insights into type VI secretion systems and their functional repertoires inmSystems · 2026Article
- The type VI secretion system of Acinetobacter: mechanisms, biology and therapeutic potential.Communications biology · 2026Review
- Characteristics, Whole Genome Analysis of a Virulent Phage from Avian-DerivedMicroorganisms · 2026Article
- TseCurrent research in microbial sciences · 2026Article
- Dissecting the antibacterial functions of the T6SS-2 cluster inApplied and environmental microbiology · 2025Article
- Impact of hcp and vgrG on Acinetobacter baumannii biofilm formation during infection of human pulmonary alveolar epithelial cells.Scientific reports · 2025Article
- A broad-spectrum anti-fungal effector dictates bacterial-fungal interkingdom interactions.PLoS pathogens · 2025Article
- Genomic profiling and experimental validation of type VI secretion system-associated proteins in Klebsiella.PLoS genetics · 2025Article
- Genomic Characteristics and Antibiotic Resistance Evolution of Vibrio cholerae O139 - Anhui Province, China, 2013-2024.China CDC weekly · 2025Article
- Article
- Engineering the T6SS of Pseudomonas for targeted delivery of antibacterial and antifungal effectors.Journal of biological engineering · 2025Article
- Not just passengers: effectors contribute to the assembly of the type VI secretion system as structural building blocks.Journal of bacteriology · 2025Review
- Article
- Delivery determinants of anmBio · 2025Article
- Fulminant necrotizing fasciitis secondary toFrontiers in medicine · 2025Article
- Infection and Genomic Characteristics ofChina CDC weekly · 2024Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The type VI secretion system (T6SS) is a spear-like nanomachine found in gram-negative pathogens for delivery of toxic effectors to neighboring bacterial and host cells. Its assembly requires a tip spike complex consisting of a VgrG-trimer, a PAAR protein, and the interacting effectors. However, how the spike controls T6SS assembly remains elusive. Here we investigated the role of three VgrG-effector pairs in Aeromonas dhakensis strain SSU, a clinical isolate with a constitutively active T6SS. By swapping VgrG tail sequences, we demonstrate that the C-terminal ~30 amino-acid tail dictates effector specificity. Double deletion of vgrG1&2 genes (VgrG3+) abolished T6SS secretion, which can be rescued by ectopically expressing chimeric VgrG3 with a VgrG1/2-tail but not the wild type VgrG3. In addition, deletion of effector-specific chaperones also severely impaired T6SS secretion, despite the presence of intact VgrG and effector proteins, in both SSU and Vibrio cholerae V52. We further show that SSU could deliver a V. cholerae effector VasX when expressing a plasmid-borne chimeric VgrG with VasX-specific VgrG tail and chaperone sequences. Pull-down analyses show that two SSU effectors, TseP and TseC, could interact with their cognate VgrGs, the baseplate protein TssK, and the key assembly chaperone TssA. Effectors TseL and VasX could interact with TssF, TssK and TssA in V. cholerae. Collectively, we demonstrate that chimeric VgrG-effector pairs could bypass the requirement of heterologous VgrG complex and propose that effector-stuffing inside the baseplate complex, facilitated by chaperones and the interaction with structural proteins, serves as a crucial structural determinant for T6SS assembly.
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