ArticleApplied and environmental microbiology2022
Heterologous Expression of Active
Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- Cofactor binding interactions with reductive dehalogenase shape cobamide utilization in organohalide-respiring bacteria.Communications biology · 2026Article
- Developing anApplied and environmental microbiology · 2026Article
- From Energy Deprivation Stress to Catalytic Adaptation of Reductive Dehalogenase TmrA for 1,1-DCA Dechlorination: An Experimental and in Silico Study.Microbial biotechnology · 2026Article
- Current Research Advances and Future Prospects on Microbial Consortia for Sustainable PFAS Remediation.International journal of molecular sciences · 2026Review
- Deciphering reductive dehalogenase specificity through targeted mutagenesis of chloroalkane reductases.Applied and environmental microbiology · 2025Article
- Evolution of pollutant biodegradation.Applied microbiology and biotechnology · 2025Review
- FromApplied and environmental microbiology · 2024Article
- Organohalide respiration: retrospective and perspective through bibliometrics.Frontiers in microbiology · 2024Article
- Editorial: Organohalide respiration: New findings in metabolic mechanisms and bioremediation applications, Volume II.Frontiers in microbiology · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reductive dehalogenases (RDases) are a family of redox enzymes that are required for anaerobic organohalide respiration, a microbial process that is useful in bioremediation. Structural and mechanistic studies of these enzymes have been greatly impeded due to challenges in RDase heterologous expression, potentially because of their cobamide-dependence. There have been a few successful attempts at RDase production in unconventional heterologous hosts, but a robust method has yet to be developed. Here we outline a novel respiratory RDase expression system using Escherichia coli. The overexpression of E. coli's cobamide transport system,
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.