ArticleACS synthetic biology2021
Engineered l-Lactate Responding Promoter System Operating in Glucose-Rich and Anoxic Environments.
Article in ACS synthetic biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- What Does Bacteria Have to Do with Cancer? The Influence of the Body's Microbiota on Cancer in Cats and Dogs.International journal of molecular sciences · 2026Review
- Engineering oncolytic bacteria as precision cancer therapeutics: design principles, therapeutic strategies, and translational perspectives.Protein & cell · 2026Review
- Engineered microbes to enable a circular economy for biodegradable plastics.Frontiers in microbiology · 2026Review
- Self-Adjusting Engineered Probiotic for Targeted Tumor Colonization and Local Therapeutics Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- EngineeredACS synthetic biology · 2025Article
- Precision tumor treatment utilizing bacteria: principles and future perspectives.Applied microbiology and biotechnology · 2025Review
- Synergistic effects of bacteria, enzymes, and cyclic mechanical stresses on the bond strength of composite restorations.Biomaterials advances · 2025Article
- Microbial biosensors for diagnostics, surveillance and epidemiology: Today's achievements and tomorrow's prospects.Microbial biotechnology · 2024Review
- EngineeredbioRxiv : the preprint server for biology · 2024Article
- Characterization of Eight Bacterial Biosensors for Microbial Diagnostic and Therapeutic Applications.ACS synthetic biology · 2022Article
- A rapid screening platform to coculture bacteria within tumor spheroids.Nature protocols · 2022Review
- Identification and characterization of L- and D-lactate-inducible systems from Escherichia coli MG1655, Cupriavidus necator H16 and Pseudomonas species.Scientific reports · 2022Article
- A rapid and standardized workflow for functional assessment of bacterial biosensors in fecal samples.Frontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria equipped with genetically encoded lactate biosensors are promising tools for biopharmaceutical production, diagnostics, and cellular therapies. However, many applications involve glucose-rich and anoxic environments, in which current whole-cell lactate biosensors show low performance. Here we engineer an optimized, synthetic lactate biosensor system by repurposing the natural LldPRD promoter regulated by the LldR transcriptional regulator. We removed glucose catabolite and anoxic repression by designing a hybrid promoter, containing LldR operators and tuned both regulator and reporter gene expressions to optimize biosensor signal-to-noise ratio. The resulting lactate biosensor, termed ALPaGA (A Lactate Promoter Operating in Glucose and Anoxia), can operate in glucose-rich, aerobic and anoxic conditions. We show that ALPaGA works reliably in the probiotic chassis
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.