ArticleGenetics2022
Single-nucleus transcriptomes reveal evolutionary and functional properties of cell types in the Drosophila accessory gland.
Article in Genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 28 citations in OpenAlex.
- Abdominal-B regulates the male seminal fluid transferome and new fecundity factors required for sperm sex peptide binding.PLoS genetics · 2026Article
- Article
- Two classes of amine/glutamate multi-transmitter neurons innervate Drosophila internal male reproductive organs.bioRxiv : the preprint server for biology · 2025Article
- Adaptive gene expression parallelism in the male reproductive tract of two Drosophila species.Genetics · 2025Article
- Phylogenetic Relatedness Does not Predict Patterns of Parallel Transcriptome Adaptation in Drosophila.Genome biology and evolution · 2025Article
- Mating system variation and gene expression in the male reproductive tract of Peromyscus mice.Molecular ecology · 2025Article
- Evidence for Transcriptomic Conservation Between the Main Cells of thebioRxiv : the preprint server for biology · 2025Article
- New perspectives on Drosophila melanogaster de novo gene origination revealed by investigation of ancient African genetic variation.Genetics · 2025Article
- Regional specialization, polyploidy, and seminal fluid transcripts in theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Cell cycle variants during Drosophila male accessory gland development.G3 (Bethesda, Md.) · 2024Article
- Evolution and genetics of accessory gland transcriptome divergence between Drosophila melanogaster and D. simulans.Genetics · 2024Article
- Decoupled evolution of theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The seminal odorant binding protein Obp56g is required for mating plug formation and male fertility ineLife · 2023Article
- Decoupled evolution of thebioRxiv : the preprint server for biology · 2023Article
- Male-derived transcripts isolated from the mated female reproductive tract in Drosophila melanogaster.G3 (Bethesda, Md.) · 2023Article
- Selection and geography shape male reproductive tract transcriptomes in Drosophila melanogaster.Genetics · 2023Article
- Article
- Population biology of accessory gland-expressed de novo genes in Drosophila melanogaster.Genetics · 2022Article
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3 authors at 1 institution in 1 country.
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Abstract
Many traits responsible for male reproduction evolve quickly, including gene expression phenotypes in germline and somatic male reproductive tissues. Rapid male evolution in polyandrous species is thought to be driven by competition among males for fertilizations and conflicts between male and female fitness interests that manifest in postcopulatory phenotypes. In Drosophila, seminal fluid proteins secreted by three major cell types of the male accessory gland and ejaculatory duct are required for female sperm storage and use, and influence female postcopulatory traits. Recent work has shown that these cell types have overlapping but distinct effects on female postcopulatory biology, yet relatively little is known about their evolutionary properties. Here, we use single-nucleus RNA-Seq of the accessory gland and ejaculatory duct from Drosophila melanogaster and two closely related species to comprehensively describe the cell diversity of these tissues and their transcriptome evolution for the first time. We find that seminal fluid transcripts are strongly partitioned across the major cell types, and expression of many other genes additionally defines each cell type. We also report previously undocumented diversity in main cells. Transcriptome divergence was found to be heterogeneous across cell types and lineages, revealing a complex evolutionary process. Furthermore, protein adaptation varied across cell types, with potential consequences for our understanding of selection on male postcopulatory traits.
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