ArticleCommunications biology2021
Infrared nanoscopy and tomography of intracellular structures.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- Infrared Near-Field Microscopy as a Platform for Nanoscale Biomedical Spectroscopy and Imaging.ACS applied materials & interfaces · 2026Review
- Colloidal Deacetylation of Chitin Nanocrystals Results in Amorphous and Patchy Chitosan Chains.ACS nano · 2026Article
- Infrared Nano-Spectroscopy of Single Cell Sections at the Ultrastructural Level.Nanophotonics (Berlin, Germany) · 2026Article
- Infrared nanoscopy for subcellular chemical imaging.QRB discovery · 2026Review
- Nanochemical Cell-Surface Evaluation in Photothermal Spectroscopic Imaging of Antimicrobial Interactions in the Model SystemAnalytical chemistry · 2025Article
- Nanoscale Mechanical Manipulation of Ultrathin SiN Membranes Enabling Infrared Near-Field Microscopy of Liquid-Immersed samples.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Nanosecond Transient IR Spectroscopy of Halorhodopsin in Living Cells.Journal of the American Chemical Society · 2024Article
- Infrared nanoimaging of neuronal ultrastructure and nanoparticle interaction with cells.Nanoscale · 2024Article
- Visualization of Nanocarriers and Drugs in Cells and Tissue.Handbook of experimental pharmacology · 2024Article
- Molecular design and synthesis of methoxy-substitued spiropyrans with photomodulated NIR-fluorescence.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- Label-free nanoscale mapping of intracellular organelle chemistry.Communications biology · 2023Article
- Toward the Next Frontiers of Vibrational Bioimaging.Chemical & biomedical imaging · 2023Review
- Scattering-type Scanning Near-Field Optical Microscopy of Polymer-Coated Gold Nanoparticles.ACS omega · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Although techniques such as fluorescence-based super-resolution imaging or confocal microscopy simultaneously gather both morphological and chemical data, these techniques often rely on the use of localized and chemically specific markers. To eliminate this flaw, we have developed a method of examining cellular cross sections using the imaging power of scattering-type scanning near-field optical microscopy and Fourier-transform infrared spectroscopy at a spatial resolution far beyond the diffraction limit. Herewith, nanoscale surface and volumetric chemical imaging is performed using the intrinsic contrast generated by the characteristic absorption of mid-infrared radiation by the covalent bonds. We employ infrared nanoscopy to study the subcellular structures of eukaryotic (Chlamydomonas reinhardtii) and prokaryotic (Escherichia coli) species, revealing chemically distinct regions within each cell such as the microtubular structure of the flagellum. Serial 100 nm-thick cellular cross-sections were compiled into a tomogram yielding a three-dimensional infrared image of subcellular structure distribution at 20 nm resolution. The presented methodology is able to image biological samples complementing current fluorescence nanoscopy but at less interference due to the low energy of infrared radiation and the absence of labeling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.