ArticleNature communications2021
Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Non-Covalent Reversibly Photoconvertible Fluorescent Tags for Wash-Free Protein Labeling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Lighting Up Biology: GFP-Inspired Fluorescent Probes for Sensing and Imaging.Journal of the American Chemical Society · 2026Article
- A characterized promoter suite enables metabolic rewiring of the acetogen Acetobacterium woodii toward ethanol production.Biotechnology for biofuels and bioproducts · 2026Article
- A color-tailored fluorogenic sensor for hydrogen peroxide.Nature chemical biology · 2026Article
- A modular biodevice assembly tool kit for combinatorial screening and benchmarking multiple cell surface display platforms in Escherichia coli.Journal of biological engineering · 2026Article
- FLEXTAG: a small and self-renewable protein labeling system for anti-fading multi-color super-resolution imaging.Nature communications · 2026Article
- Vision of Life's Code: Molecular Probe Nanoarchitectonics for Deep RNA/DNA Illumination.ACS nano · 2026Review
- Fast, Bright, and Reversible Fluorescent Labeling of Rhodamine-Binding Proteins.Journal of the American Chemical Society · 2026Article
- Reversible Chemogenetic Fluorescence Labeling with pFAST inmicroPublication biology · 2026Article
- Bathochromic Shift via C=O to C=S Substitution: A Far-Red Fluorogen for Multiplexed FLIM with FAST Fluorogen-Activating Protein.International journal of molecular sciences · 2025Article
- Unequal mitochondrial segregation promotes asymmetric fates during neurogenesis.Nature communications · 2025Article
- Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes.Nature communications · 2025Article
- FLEXTAG: A Small and Self-renewable Protein Labeling System for Anti-fading Multicolor Super-resolution Imaging.bioRxiv : the preprint server for biology · 2025Article
- Protein Engineering for Spatiotemporally Resolved Cellular Monitoring.Annual review of analytical chemistry (Palo Alto, Calif.) · 2025Review
- A tunable and versatile chemogenetic near-infrared fluorescent reporter.Nature communications · 2025Article
- Fluorescent labeling strategies for molecular bioimaging.Biophysical reports · 2025Review
- Multipartite Fluorogenic Sensors for Monitoring Tyrosine Phosphatase Activity.Chembiochem : a European journal of chemical biology · 2024Article
- Multiplexed In Vivo Imaging with Fluorescence Lifetime-Modulating Tags.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism.Annual review of biophysics · 2024Review
- Structural Analysis of the Large Stokes Shift Red Fluorescent Protein tKeima.Molecules (Basel, Switzerland) · 2024Article
Corrections and comments
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Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biocompatible fluorescent reporters with spectral properties spanning the entire visible spectrum are indispensable tools for imaging the biochemistry of living cells and organisms in real time. Here, we report the engineering of a fluorescent chemogenetic reporter with tunable optical and spectral properties. A collection of fluorogenic chromophores with various electronic properties enables to generate bimolecular fluorescent assemblies that cover the visible spectrum from blue to red using a single protein tag engineered and optimized by directed evolution and rational design. The ability to tune the fluorescence color and properties through simple molecular modulation provides a broad experimental versatility for imaging proteins in live cells, including neurons, and in multicellular organisms, and opens avenues for optimizing Förster resonance energy transfer (FRET) biosensors in live cells. The ability to tune the spectral properties and fluorescence performance enables furthermore to match the specifications and requirements of advanced super-resolution imaging techniques.
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Registered trials
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