ArticleHorticulture research2021
Transcriptional regulation of flavonoid biosynthesis in Artemisia annua by AaYABBY5.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Multi-target synergistic mechanisms of flavonoid compounds from traditional Chinese medicine in non-alcoholic fatty liver disease: insights for human and veterinary medicine.Frontiers in veterinary science · 2026Pooled it
- A Novel CsYABBY3-CsAS1 Feedback Loop Coordinates Trichome Differentiation and Cannabinoid Biosynthesis in Cannabis sativa L.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- SmYABBY1, a Light-Inducible Transcription Factor, Positively Regulates Anthocyanin Biosynthesis in Eggplant (International journal of molecular sciences · 2026Article
- CcMYB24 redirects flavonoid metabolic flux toward flavonols by activating CcFLS and CcCHI to drive leaf variegation in Cymbidium.BMC plant biology · 2026Article
- CsYABBY1 and CsMYB114 Enhance Acquired Drought Tolerance by Mediating Flavonoid Biosynthesis in Camellia sinensis.Plant biotechnology journal · 2026Article
- A chromosome level genome, as well as transcriptomes and metabolomes, insights into genome evolution and the biosynthesis of kaempferol and kaempferol derivatives inFrontiers in plant science · 2026Article
- Integrative metabolomic and transcriptomic analyses reveal flavonoid biosynthesis pathway in Eupatorium lindleyanum.Scientific reports · 2025Article
- Transcriptome and metabolome analysis of Cycas bifida response to low-temperature stress.BMC plant biology · 2025Article
- Identification of the YABBY Gene Family inBiology · 2025Article
- Organ-specific transcriptional and untargeted metabolome analysis demystifies molecular insights and regulation of Gallic acid and flavonoid biosynthesis in Bergenia ciliata.Functional & integrative genomics · 2025Article
- Transcription Factor Analysis of Rhodophytes Suggests Trihelix Transcription Factors Across the Florideophyceae.Plants (Basel, Switzerland) · 2025Article
- A model for the adaptation of Euryale ferox leaves to aquatic environments through EfCGT1-controlled flavonoid C-glycoside-specific accumulation in epidermis cells.Plant biotechnology journal · 2025Article
- Amino acid metabolism pathways as key regulators of nitrogen distribution in tobacco: insights from transcriptome and WGCNA analyses.BMC plant biology · 2025Article
- Metabolomics combined with transcriptomics reveals the formation mechanism of different leaf colors ofFrontiers in plant science · 2025Article
- Unlocking the potential of flavonoid biosynthesis through integrated metabolic engineering.Frontiers in plant science · 2025Review
- Multidimensional regulation of transcription factors: decoding the comprehensive signals of plant secondary metabolism.Frontiers in plant science · 2025Review
- Genome-Wide Identification of the Lectin Receptor-like Kinase Gene Family inInternational journal of molecular sciences · 2024Article
- The influence of spatial distribution and transcriptional regulation of secondary metabolites on the bioactivities ofHeliyon · 2024Article
- The gap-free genome and multi-omics analysis ofHorticulture research · 2024Article
- Characterization of YABBY transcription factors in Osmanthus fragrans and functional analysis of OfYABBY12 in floral scent formation and leaf morphology.BMC plant biology · 2024Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Artemisia annua is a medicinal plant rich in terpenes and flavonoids with useful biological activities such as antioxidant, anticancer, and antimalarial activities. The transcriptional regulation of flavonoid biosynthesis in A. annua has not been well-studied. In this study, we identified a YABBY family transcription factor, AaYABBY5, as a positive regulator of anthocyanin and total flavonoid contents in A. annua. AaYABBY5 was selected based on its similar expression pattern to the phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), and flavonol synthase (FLS) genes. A transient dual-luciferase assay in Nicotiana bethamiana with the AaYABBY5 effector showed a significant increase in the activity of the downstream LUC gene, with reporters AaPAL, AaCHS, AaCHI, and AaUFGT. The yeast one-hybrid system further confirmed the direct activation of these promoters by AaYABBY5. Gene expression analysis of stably transformed AaYABBY5 overexpression, AaYABBY5 antisense, and control plants revealed a significant increase in the expression of AaPAL, AaCHS, AaCHI, AaFLS, AaFSII, AaLDOX, and AaUFGT in AaYABBY5 overexpression plants. Moreover, their total flavonoid content and anthocyanin content were also found to increase. AaYABBY5 antisense plants showed a significant decrease in the expression of flavonoid biosynthetic genes, as well as a decrease in anthocyanin and total flavonoid contents. In addition, phenotypic analysis revealed deep purple-pigmented stems, an increase in the leaf lamina size, and higher trichome densities in AaYABBY5 overexpression plants. Together, these data proved that AaYABBY5 is a positive regulator of flavonoid biosynthesis in A. annua. Our study provides candidate transcription factors for the improvement of flavonoid concentrations in A. annua and can be further extended to elucidate its mechanism of regulating trichome development.
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