ArticleHorticulture research2021
Disruption of the bHLH transcription factor Abnormal Tapetum 1 causes male sterility in watermelon.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Molecular Targets and Trait Innovation in CRISPR-Edited Vegetable Crops: An Up-to-Date Review (2020-2026).Plants (Basel, Switzerland) · 2026Review
- The KNOX transcription factor ClSP activates ClAPRR2 to regulate dark green stripe formation in watermelon.Plant biotechnology journal · 2025Article
- Construction of a co-expression network and mining of core genes for genic male sterility in sweet pepper based on transcriptome and WGCNA analysis.Frontiers in plant science · 2025Article
- Mutations in a Leucine-Rich Repeat Receptor-Like Kinase gene result in male sterility and reduction in the number and size of fruit warts in cucumber (Cucumis sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Application of genome editing in plant reproductive biology: recent advances and challenges.Plant reproduction · 2024Review
- Identification of differentially expressed miRNAs between male sterile and fertile floral buds in watermelon (3 Biotech · 2024Article
- Article
- Genome-wide identification of bHLH transcription factors and functional analysis in salt gland development of the recretohalophyte sea lavender (Horticulture research · 2024Article
- Identification of male-fertility geneFrontiers in plant science · 2024Article
- An affordable and convenient diagnostic marker to identify male and female hop plants.G3 (Bethesda, Md.) · 2023Article
- Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses.International journal of molecular sciences · 2023Review
- Current insights and advances into plant male sterility: new precision breeding technology based on genome editing applications.Frontiers in plant science · 2023Review
- Whole-transcriptome analyses identify key differentially expressed mRNAs, lncRNAs, and miRNAs associated with male sterility in watermelon.Frontiers in plant science · 2023Article
- Genic male and female sterility in vegetable crops.Horticulture research · 2023Article
- Editing melon eIF4E associates with virus resistance and male sterility.Plant biotechnology journal · 2022Article
- The bHLH1-DTX35/DFR module regulates pollen fertility by promoting flavonoid biosynthesis inHorticulture research · 2022Article
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
Abstract
Although male sterility has been identified as a useful trait for hybrid vigor utilization and hybrid seed production, its underlying molecular mechanisms in Cucurbitaceae species are still largely unclear. Here, a spontaneous male-sterile watermelon mutant, Se18, was reported to have abnormal tapetum development, which resulted in completely aborted pollen grains. Map-based cloning demonstrated that the causal gene Citrullus lanatus Abnormal Tapetum 1 (ClATM1) encodes a basic helix-loop-helix (bHLH) transcription factor with a 10-bp deletion and produces a truncated protein without the bHLH interaction and functional (BIF) domain in Se18 plants. qRT-PCR and RNA in situ hybridization showed that ClATM1 is specifically expressed in the tapetum layer and in microsporocytes during stages 6-8a of anther development. The genetic function of ClATM1 in regulating anther development was verified by CRISPR/Cas9-mediated mutagenesis. Moreover, ClATM1 was significantly downregulated in the Se18 mutant, displaying a clear dose effect at the transcriptional level. Subsequent dual-luciferase reporter, β-glucuronidase (GUS) activity, and yeast one-hybrid assays indicated that ClATM1 could activate its own transcriptional expression through promoter binding. Collectively, ClATM1 is the first male sterility gene cloned from watermelon, and its self-regulatory activity provides new insights into the molecular mechanism underlying anther development in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.