Evidence map›Paper›PMID 34845372›Full record

ArticleNature biotechnology2022

Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging.

Kanuj Mishra, Juan Pablo Fuenzalida-Werner, Francesca Pennacchietti, Robert Janowski, Andriy Chmyrov, Yuanhui Huang, Christian Zakian, Uwe Klemm, Ilaria Testa, Dierk Niessing and 2 more

Open access · hybridAbstract read
In one paragraph

Article in Nature biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
3.4field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 58 citations in OpenAlex.

  1. Article
  2. Article
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  4. Article
  5. Photon-to-Heat Energy Harvesting Fluorescent Protein Coatings for Thermoelectrics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Chemigenetic Far-Red Labels and CaJournal of the American Chemical Society · 2024
    Article
  14. Review
  15. Review
  16. Article
  17. Article
  18. Review
  19. Photoswitching alters fluorescence readout of jGCaMP8 CaProceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Kanuj Mishra *Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany.ORCID http://orcid.org/0000-0002-2462-8834
Juan Pablo Fuenzalida-Werner *Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany.ORCID http://orcid.org/0000-0001-6651-3851
Francesca Pennacchietti *Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.
Robert Janowski *Intracellular Transport and RNA Biology Group, Institute of Structural Biology, Helmholtz Zentrum München, Neuherberg, Germany.ORCID http://orcid.org/0000-0002-9940-2143
Andriy ChmyrovInstitute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany.ORCID http://orcid.org/0000-0003-0265-019X
Yuanhui HuangInstitute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany.ORCID http://orcid.org/0000-0002-4774-7449
Christian ZakianInstitute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany.
Uwe KlemmInstitute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany.
Ilaria TestaScience for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.ORCID http://orcid.org/0000-0003-4005-4997
Dierk NiessingIntracellular Transport and RNA Biology Group, Institute of Structural Biology, Helmholtz Zentrum München, Neuherberg, Germany.
Vasilis NtziachristosInstitute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany.ORCID http://orcid.org/0000-0002-9988-0233
Andre C StielInstitute of Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany. andre.stiel@helmholtz-muenchen.de.ORCID http://orcid.org/0000-0001-8675-6797
Helmholtz Zentrum München · DEScience for Life Laboratory · SEUniversität Ulm · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reversibly photo-switchable proteins are essential for many super-resolution fluorescence microscopic and optoacoustic imaging methods. However, they have yet to be used as sensors that measure the distribution of specific analytes at the nanoscale or in the tissues of live animals. Here we constructed the prototype of a photo-switchable Ca

Indexed as

Photoacoustic TechniquesAnimalsCell LineMiceMicroscopy, Fluorescence

Identifiers

PMID34845372
PMCPMC9005348
OpenAlexW3217060248

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.