ArticleBMC plant biology2021
Construction of yeast one-hybrid library and screening of transcription factors regulating LhMYBSPLATTER expression in Asiatic hybrid lilies (Lilium spp.).
Article in BMC plant biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 27 citations in OpenAlex.
- MicroRNA858a antagonistically regulates plant response to concurrent biotic and abiotic stresses.Plant signaling & behavior · 2026Article
- Construction of a Yeast Two-Hybrid Library for Lilium × formolongi and Screening for Proteins Interacting with LfSVP.Molecular biotechnology · 2026Article
- Effect of canopy height and tree shape on anthocyanins accumulation in wine grape.Food chemistry. Molecular sciences · 2026Article
- Integrated multi-omics analysis reveals the regulatory mechanism of cryptochrome 1- mediated anthocyanin biosynthesis in Populus trichocarpa.BMC plant biology · 2026Article
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- Construction of a Normalized Library and Screening of Transcriptional Regulators of theMicroorganisms · 2026Article
- Candidate promoter-associated factors ofFrontiers in plant science · 2026Article
- Construction of Yeast One-Hybrid Library ofBiomolecules · 2025Article
- Screening of MYB1R1 interaction withPeerJ · 2025Article
- Identification and Characterization of EIN3/EIL Transcription Factor Family Members inInternational journal of molecular sciences · 2024Article
- Multifaceted roles of LhWRKY44 in promoting anthocyanin accumulation in Asiatic hybrid lilies (Horticulture research · 2023Article
- Construction of Yeast One-Hybrid Library ofJournal of fungi (Basel, Switzerland) · 2023Article
- Functional analysis of PagNAC045 transcription factor that improves salt and ABA tolerance in transgenic tobacco.BMC plant biology · 2022Article
- ERF subfamily transcription factors and their function in plant responses to abiotic stresses.Frontiers in plant science · 2022Review
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Authors and funding
9 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundAnthocyanins, which belong to flavonoids, are widely colored among red-purple pigments in the Asiatic hybrid lilies (Lilium spp.). Transcription factor (TF) LhMYBSPLATTER (formerly known as LhMYB12-Lat), identified as the major kernel protein, regulating the anthocyanin biosynthesis pathway in 'Tiny Padhye' of Tango Series cultivars, which the pigmentation density is high in the lower half of tepals and this patterning is of exceptional ornamental value. However, the research on mechanism of regulating the spatial and temporal expression differences of LhMYBSPLATTER, which belongs to the R2R3-MYB subfamily, is still not well established. To explore the molecular mechanism of directly related regulatory proteins of LhMYBSPLATTER in the anthocyanin pigmentation, the yeast one-hybrid (Y1H) cDNA library was constructed and characterized.
resultsIn this study, we describe a yeast one-hybrid library to screen transcription factors that regulate LhMYBSPLATTER gene expression in Lilium, with the library recombinant efficiency of over 98%. The lengths of inserted fragments ranged from 400 to 2000 bp, and the library capacity reached 1.6 × 10
conclusionA yeast one-hybrid library of lily was successfully constructed in the tepals for the first time. Seven candidate TFs of LhMYBSPLATTER were screened, which may provide a theoretical basis for the study of floral pigmentation.
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