ArticleJournal of lipid research2022
Loss of plasma membrane lipid asymmetry can induce ordered domain (raft) formation.
Article in Journal of lipid research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Paraformaldehyde-Induced Vesiculation Preserves Native Membrane Physiological States Critical for T‑Cell Activation.ACS omega · 2026Article
- Protein-enhanced small molecule disruptors of ordered membrane domains.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- ATP8B1-TMEM30B Flippase Activity Maintains Stereocilia Lipid Asymmetry Required for Hearing.bioRxiv : the preprint server for biology · 2026Article
- Asymmetry and heterogeneity in the plasma membrane.Biophysical journal · 2026Article
- Influenza viral infection at the plasma membrane is restricted by lipid composition.Journal of virology · 2025Article
- Effect of a scramblase activator upon lipid and probe scrambling and membrane domain formation in HEK 293T cells.Faraday discussions · 2025Article
- The Importance of Bilayer Asymmetry in Biological Membranes: Insights from Model Membranes.Membranes · 2025Review
- Influence of the glycocalyx on the size and mechanical properties of plasma membrane-derived vesicles.Soft matter · 2025Article
- Cellular Output and Physicochemical Properties of the Membrane-Derived Vesicles Depend on Chemical Stimulants.ACS applied materials & interfaces · 2024Article
- Article
- Lipid-driven interleaflet coupling of plasma membrane order regulates FcεRI signaling in mast cells.Biophysical journal · 2024Article
- Using the yeast vacuole as a system to test the lipidic drivers of membrane heterogeneity in living cells.Methods in enzymology · 2024Article
- Experimentally determined leaflet-leaflet phase diagram of an asymmetric lipid bilayer.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Ordered Domain (Raft) Formation in Asymmetric Vesicles and Its Induction upon Loss of Lipid Asymmetry in Artificial and Natural Membranes.Membranes · 2022Article
- Direct quantification of ligand-induced lipid and protein microdomains with distinctive signaling properties.ChemSystemsChem · 2022Article
- Transbilayer Coupling of Lipids in Cells Investigated by Imaging Fluorescence Correlation Spectroscopy.The journal of physical chemistry. B · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
In some cases, lipids in one leaflet of an asymmetric artificial lipid vesicle suppress the formation of ordered lipid domains (rafts) in the opposing leaflet. Whether this occurs in natural membranes is unknown. Here, we investigated this issue using plasma membrane vesicles (PMVs) from rat leukemia RBL-2H3 cells. Membrane domain formation and order was assessed by fluorescence resonance energy transfer and fluorescence anisotropy. We found that ordered domains in PMVs prepared from cells by N-ethyl maleimide (NEM) treatment formed up to ∼37°C, whereas ordered domains in symmetric vesicles formed from the extracted PMV lipids were stable up to 55°C, indicating the stability of ordered domains was substantially decreased in intact PMVs. This behavior paralleled lesser ordered domain stability in artificial asymmetric lipid vesicles relative to the corresponding symmetric vesicles, suggesting intact PMVs exhibit some degree of lipid asymmetry. This was supported by phosphatidylserine mislocalization on PMV outer leaflets as judged by annexin binding, which indicated NEM-induced PMVs are much more asymmetric than PMVs formed by dithiothreitol/paraformaldehyde treatment. Destroying asymmetry by reconstitution of PMVs using detergent dilution also showed stabilization of domain formation, even though membrane proteins remained associated with reconstituted vesicles. Similar domain stabilization was observed in artificial asymmetric lipid vesicles after destroying asymmetry via detergent reconstitution. Proteinase K digestion of proteins had little effect on domain stability in NEM PMVs. We conclude that loss of PMV lipid asymmetry can induce ordered domain formation. The dynamic control of lipid asymmetry in cells may regulate domain formation in plasma membranes.
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