ArticlePLoS genetics2021
Dynamic genome plasticity during unisexual reproduction in the human fungal pathogen Cryptococcus deneoformans.
Article in PLoS genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- Host skin lipids triggerbioRxiv : the preprint server for biology · 2026Article
- Genomic and Ecological Flexibility Shape the Global Distribution of a Black Fungus.Environmental microbiology · 2026Article
- Homeodomain protein Sxi1α independently controls cell-cell fusion and gene expression during sexual reproduction in Cryptococcus deneoformans.PLoS genetics · 2026Article
- Aneuploidy, polyploidy, and loss of heterozygosity distinguish serial bloodstream isolates ofbioRxiv : the preprint server for biology · 2025Article
- Unisexual reproduction in the global human fungal pathogenProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Unisexual reproduction in the global human fungal pathogenbioRxiv : the preprint server for biology · 2025Article
- Homeodomain protein Sxi1α independently controls cell-cell fusion and gene expression during sexual reproduction inbioRxiv : the preprint server for biology · 2025Article
- Evolution of antifungal resistance in the environment.Nature microbiology · 2025Review
- Adaptation of the tetracycline-repressible system for modulating the expression of essential genes inmSphere · 2025Article
- Understanding the clinical and environmental drivers of antifungal resistance in the One Health context.Microbiology (Reading, England) · 2024Review
- Comparative genomics of the closely related fungal genera Cryptococcus and Kwoniella reveals karyotype dynamics and suggests evolutionary mechanisms of pathogenesis.PLoS biology · 2024Article
- Behind the scenes: Centromere-driven genomic innovations in fungal pathogens.PLoS pathogens · 2024Article
- Comparative genomics ofbioRxiv : the preprint server for biology · 2024Article
- Genetic Diversity of Human Fungal Pathogens.Current clinical microbiology reports · 2023Article
- Genome-wide analysis of heat stress-stimulated transposon mobility in the human fungal pathogenProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Regulatory basis for reproductive flexibility in a meningitis-causing fungal pathogen.Nature communications · 2022Article
- Ploidy changes in human fungal pathogens: Going beyond sexual reproduction.PLoS pathogens · 2022Article
- Identification and Characterization of an Intergenic "Safe Haven" Region in Human Fungal PathogenJournal of fungi (Basel, Switzerland) · 2022Article
- Genetic analysis of Hsp90 function in Cryptococcus neoformans highlights key roles in stress tolerance and virulence.Genetics · 2022Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Genome copy number variation occurs during each mitotic and meiotic cycle and it is crucial for organisms to maintain their natural ploidy. Defects in ploidy transitions can lead to chromosome instability, which is a hallmark of cancer. Ploidy in the haploid human fungal pathogen Cryptococcus neoformans is exquisitely orchestrated and ranges from haploid to polyploid during sexual development and under various environmental and host conditions. However, the mechanisms controlling these ploidy transitions are largely unknown. During C. deneoformans (formerly C. neoformans var. neoformans, serotype D) unisexual reproduction, ploidy increases prior to the onset of meiosis, can be independent from cell-cell fusion and nuclear fusion, and likely occurs through an endoreplication pathway. To elucidate the molecular mechanisms underlying this ploidy transition, we identified twenty cell cycle-regulating genes encoding cyclins, cyclin-dependent kinases (CDK), and CDK regulators. We characterized four cyclin genes and two CDK regulator genes that were differentially expressed during unisexual reproduction and contributed to diploidization. To detect ploidy transition events, we generated a ploidy reporter, called NURAT, which can detect copy number increases via double selection for nourseothricin-resistant, uracil-prototrophic cells. Utilizing this ploidy reporter, we showed that ploidy transition from haploid to diploid can be detected during the early phases of unisexual reproduction. Interestingly, selection for the NURAT reporter revealed several instances of segmental aneuploidy of multiple chromosomes, which conferred azole resistance in some isolates. These findings provide further evidence of ploidy plasticity in fungi with significant biological and public health implications.
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