ArticleJournal of visualized experiments : JoVE2021
Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles.
Article in Journal of visualized experiments : JoVE, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 19 citations in OpenAlex.
- Lipid composition effects on the number and size of liposomes formed by the inverted emulsion method.Biophysical journal · 2026Article
- Tau-Driven Coordination of Microtubule-Actin Crosstalk in Cell-Sized Vesicles.bioRxiv : the preprint server for biology · 2025Article
- Strategy for Generating Giant Unilamellar Vesicles with Tunable Size Using the Modified cDICE Method.ACS synthetic biology · 2025Article
- Light-Triggered Protease-Mediated Release of Actin-Bound Cargo from Synthetic Cells.Advanced biology · 2025Article
- Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles.Advanced biology · 2025Article
- Challenges in observing transcription-translation for bottom-up synthetic biology.QRB discovery · 2025Review
- Rearrangement of GUV-confined actin networks in response to micropipette aspiration.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Reconstitution of the Bacterial Glutamate Receptor Channel by Encapsulation of a Cell-Free Expression System.Journal of visualized experiments : JoVE · 2024Article
- Hybrid Vesicles Enable Mechano-Responsive Hydrogel Degradation.Angewandte Chemie (International ed. in English) · 2023Article
- Branched actin cortices reconstituted in vesicles sense membrane curvature.Biophysical journal · 2023Article
- Differential regulation of GUV mechanics via actin network architectures.Biophysical journal · 2023Article
- Methods to mechanically perturb and characterize GUV-based minimal cell models.Computational and structural biotechnology journal · 2023Review
- Membrane stretching activates calcium permeability of a putative channel Pkd2 during fission yeast cytokinesis.Molecular biology of the cell · 2022Article
- Studying actin-induced cell shape changes using Giant Unilamellar Vesicles and reconstituted actin networks.Biochemical Society transactions · 2022Review
- Actomyosin-Driven Division of a Synthetic Cell.ACS synthetic biology · 2022Review
- Reconstitution of Membrane-tethered Minimal Actin Cortices on Supported Lipid Bilayers.Journal of visualized experiments : JoVE · 2022Article
- Article
- Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction.Frontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Giant unilamellar vesicles (GUVs) are frequently used as models of biological membranes and thus are a great tool to study membrane-related cellular processes in vitro. In recent years, encapsulation within GUVs has proven to be a helpful approach for reconstitution experiments in cell biology and related fields. It better mimics confinement conditions inside living cells, as opposed to conventional biochemical reconstitution. Methods for encapsulation inside GUVs are often not easy to implement, and success rates can differ significantly from lab to lab. One technique that has proven to be successful for encapsulating more complex protein systems is called continuous droplet interface crossing encapsulation (cDICE). Here, a cDICE-based method is presented for rapidly encapsulating cytoskeletal proteins in GUVs with high encapsulation efficiency. In this method, first, lipid-monolayer droplets are generated by emulsifying a protein solution of interest in a lipid/oil mixture. After being added into a rotating 3D-printed chamber, these lipid-monolayered droplets then pass through a second lipid monolayer at a water/oil interface inside the chamber to form GUVs that contain the protein system. This method simplifies the overall procedure of encapsulation within GUVs and speeds up the process, and thus allows us to confine and observe the dynamic evolution of network assembly inside lipid bilayer vesicles. This platform is handy for studying the mechanics of cytoskeleton-membrane interactions in confinement.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.