Evidence map›Paper›PMID 34841745›Full record

ArticleJournal of veterinary science2022

Protective efficacy of a novel multivalent vaccine in the prevention of diarrhea induced by enterotoxigenic

Hong Zhao, Yongping Xu, Gen Li, Xin Liu, Xiaoyu Li, Lili Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Hong ZhaoSchool of Bioengineering, Dalian University of Technology, Dalian 116024, China.ORCID https://orcid.org/0000-0002-9065-2103
Yongping XuSchool of Bioengineering, Dalian University of Technology, Dalian 116024, China.ORCID https://orcid.org/0000-0002-3946-9223
Gen LiSchool of Bioengineering, Dalian University of Technology, Dalian 116024, China.ORCID https://orcid.org/0000-0001-8449-8878
Xin LiuSchool of Bioengineering, Dalian University of Technology, Dalian 116024, China.ORCID https://orcid.org/0000-0001-7775-2356
Xiaoyu LiSchool of Bioengineering, Dalian University of Technology, Dalian 116024, China.ORCID https://orcid.org/0000-0002-0770-4735
Lili WangSchool of Bioengineering, Dalian University of Technology, Dalian 116024, China. wanglili@dlut.edu.cn.ORCID https://orcid.org/0000-0002-6621-404X
Dalian University of Technology · CN

Funding

Fundamental Research Funds for the Central Universities DUT20LK29National Key Research and Development Program of China 2017YFD0500601National Natural Science Foundation of China 31701719
6 · The paper itself

Abstract

backgroundEnterotoxigenic

objectivesOur study aimed to develop a multivalent ETEC vaccine targeting major virulence factors of ETEC, including enterotoxins and fimbriae.

methodsSLS (STa-LTB-STb) recombinant enterotoxin and fimbriae proteins (F4, F5, F6, F18, and F41) were prepared to develop a multivalent vaccine. A total of 65 mice were immunized subcutaneously by vaccines and phosphate-buffered saline (PBS). The levels of specific immunoglobulin G (IgG) and pro-inflammatory cytokines were determined at 0, 7, 14 and 21 days post-vaccination (dpv). A challenge test with a lethal dose of ETEC was performed, and the survival rate of the mice in each group was recorded. Feces and intestine washes were collected to measure the concentrations of secretory immunoglobulin A (sIgA).

resultsAnti-SLS and anti-fimbriae-specific IgG in serums of antigen-vaccinated mice were significantly higher than those of the control group. Immunization with the SLS enterotoxin and multivalent vaccine increased interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) concentrations. Compared to diarrheal symptoms and 100% death of mice in the control group, mice inoculated with the multivalent vaccine showed an 80% survival rate without any symptom of diarrhea, while SLS and fimbriae vaccinated groups showed 60 and 70% survival rates, respectively.

conclusionsBoth SLS and fimbriae proteins can serve as vaccine antigens, and the combination of these two antigens can elicit stronger immune responses. The results suggest that the multivalent vaccine can be successfully used for preventing ETEC in important livestock.

Indexed as

DiarrheaEscherichia coli InfectionsAnimalsAntibodies, BacterialDisease Models, AnimalEnterotoxigenic Escherichia coliEnterotoxinsEscherichia coli ProteinsEscherichia coli VaccinesFimbriae ProteinsImmunoglobulin AImmunoglobulin GMiceVaccines, CombinedAntibodies, BacterialEnterotoxinsEscherichia coli ProteinsEscherichia coli VaccinesFimbriae ProteinsImmunoglobulin AImmunoglobulin GVaccines, CombinedEnterotoxigenic Escherichia coliImmune responseMiceSmall intestineVaccine

Identifiers

PMID34841745
PMCPMC8799940
OpenAlexW3206600916

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.