Evidence map›Paper›PMID 34841077›Full record

ArticleClinical mass spectrometry (Del Mar, Calif.)2019

Rapid method towards proteomic analysis of dried blood spots by MALDI mass spectrometry.

Grace M Samenuk, Andrea R Kelley, George Perry, Stephan B H Bach

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical mass spectrometry (Del Mar, Calif.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact, top 98% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
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  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Grace M SamenukDepartment of Chemistry, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, United States.
Andrea R KelleyDepartment of Chemistry, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, United States.
George PerryDepartment of Biology, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, United States.
Stephan B H BachDepartment of Chemistry, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, United States.
The University of Texas at San Antonio · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neonatal dried blood spots (DBS) are routinely utilized in the clinical setting as a diagnostic tool for various genetic disorders and infectious diseases. DBS allow for minimally invasive, small volume blood collection and are stored at room temperature. Neonatal whole blood and serum samples can be important in determining genetic risk factors and predicting infantile disease; however, at the present time, limited methods exist for rapidly analyzing DBS samples for their proteomic profile, years after samples have been collected. A novel method is presented for the extraction and analysis of target proteins and peptides from neonatal DBS using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Extraction parameters were optimized to achieve ideal signal intensity and resolution to obtain protein identifications. Samples were extracted from filter paper with 0.1% TFA in H

Indexed as

CHCA, α-cyano-4-hydroxycinnamic acidDBSDBS, dried blood spotsDried blood spotsEtOH, ethanolHPLC, high performance liquid chromatographyITO, indium-tin-oxideLC–MS/MS, liquid chromatography tandem mass spectrometryMALDIMALDI-TOF, matrix-assisted laser desorption/ionization time-of-flightMass spectrometryMS, mass spectrometrym/z, mass-to-chargeNBS, newborn screeningNeonatal screeningPKU, phenylketonuriaSA, sinapinic acidSLE, solid-liquid extractionTFA, trifluoroacetic acid

Identifiers

PMID34841077
PMCPMC8620524
OpenAlexW2921095394

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.