Evidence map›Paper›PMID 34840962›Full record

ArticleMethodsX2021

Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2.

Jiangshan Wang, Andres Dextre, Ana Pascual-Garrigos, Josiah Levi Davidson, Darby McChesney, Jordan Seville, Mohit S Verma

Open access · goldAbstract read
In one paragraph

Article in MethodsX, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.9field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 35 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jiangshan WangDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA.
Andres DextreDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA.
Ana Pascual-GarrigosDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA.
Josiah Levi DavidsonDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA.
Darby McChesneyPortaScience, Inc., Moorestown, NJ 08057, USA.
Jordan SevillePortaScience, Inc., Moorestown, NJ 08057, USA.
Mohit S VermaDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA.
Purdue University West Lafayette · USPortaScience (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused unprecedented damage to the global economy. Diagnostic testing is a key factor in limiting virus transmission and safeguarding public health. We present the fabrication process of a paper-based device that uses reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect SARS-CoV-2 in complex matrices by providing a colorimetric response apparent to the naked eye. Because of LAMP's functionality, this device just requires a simple heat source (e.g., water bath, incubator), it can be deployed in resource-constrained areas and can be used as a supplement to current point-of-care (POC) and community testing procedures. Since the test is based on nucleic acids, the testing platform itself lends to further applications including food safety monitoring, animal diagnostics, etc. simply by changing the specific primers.•We developed a platform capable of on-paper detection of SARS-CoV-2 using colorimetric reporters that produce responses visible to the naked eye.•The platform is easily reconfigurable to target different pathogens by changing the primer sets, and multiplexing is possible by adding additional reaction sites to the device.

Indexed as

Colorimetric LAMPMicrofluidic paper-based analytical devicesPaper-based diagnosticsSARS-CoV-2

Identifiers

PMID34840962
PMCPMC8604794
OpenAlexW3216395699

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.