ArticleVeterinary world2021
The potential of sperm bovine protamine as a protein marker of semen production and quality at the National Artificial Insemination Center of Indonesia.
Article in Veterinary world, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Unveiling novel insights into the role and characteristics of sperm heat shock protein 70 in indigenous Donggala bulls: implications for molecular-based motility markers.Archives animal breeding · 2026Article
- Age-related differences in sperm chromatin packaging and protein profiles based on SDS-PAGE in Bali bulls (Journal of advanced veterinary and animal research · 2026Article
- Frozen semen quality of Pasundan bulls with different individual variations.Open veterinary journal · 2025Article
- Reduction of post-thawed sperm quality of Kacang goat using the addition of epigallocatechin-3-gallate into the skim milk-egg yolk extender.Open veterinary journal · 2025Article
- A-Kinase Anchor Protein 4 (proAKAP4): Protein Molecule-Based Fertility Marker of Indonesian Dairy Bull and Its Correlation With Frozen-Thawed Sperm Quality.Veterinary medicine international · 2025Article
- The frozen-thawed sperm protein of Indonesian Toraya buffaloes is significantly associated with sperm kinematics, acrosome integrity, and mitochondrial membrane potential.Journal of advanced veterinary and animal research · 2024Article
- Correlation of sperm motility, acrosome integrity, protamine deficiency, and DNA fragmentation in proven and unproven Friesian Holstein bulls.Journal of advanced veterinary and animal research · 2024Article
- Sperm HSP70: may not be an age-dependent gene but is associated with field fertility in Bali bulls (Animal reproduction · 2024Article
- Identification of heat shock protein70-2 and protamine-1 mRNA, proteins, and analyses of their association with fertility using frozen-thawed sperm in Madura bulls.Animal bioscience · 2023Article
- Sperm Head Morphology Alterations Associated with Chromatin Instability and Lack of Protamine Abundance in Frozen-Thawed Sperm of Indonesian Local Bulls.Animals : an open access journal from MDPI · 2023Article
- Bovine sperm HSP-70 molecules: a potential cryo-tolerance marker associated with semen quality and fertility rate.Frontiers in veterinary science · 2023Article
- Sperm Transcriptome Analysis Accurately Reveals Male Fertility Potential in Livestock.Animals : an open access journal from MDPI · 2022Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimProtamine (PRM) is the major protein in the sperm nucleus and plays an essential role in its normal function. Moreover, PRM has great potential as a protein marker of semen production and quality. This study aimed to assess the potential of sperm bovine PRM as a protein marker of semen production and quality in bulls at the National Artificial Insemination (AI) Center of Indonesia. MATERIALS AND
methodsThe semen production capacity of each bull was collected from frozen semen production data at the Singosari AI Center for 6 months, and was then divided into two groups (high and low). A total of 440 frozen semen straws from six Limousin (LIM), six Friesian Holstein (FH), six Peranakan Ongole (PO), and four Aceh bulls aged 4-5 years were used in the study. The frozen semen was used to measure the concentration of PRM1, PRM2, and PRM3 using the enzyme immunoassay method. The frozen semen was also used to assess the quality of the semen, including progressive motility (PM) through computer-assisted semen analysis, sperm viability through eosin-nigrosin analysis, and the DNA fragmentation index through Acridine Orange staining.
resultsPRM1 was significantly higher in all bull breeds included in the study (p<0.00), followed by PRM2 (p<0.00) and PRM3 (p<0.00). PRM1 significantly affected semen production in LIM, FH, PO, and Aceh bulls (p<0.05). Moreover, PRM2 significantly affected semen production only in FH and Aceh bulls (p<0.05), whereas PRM3 affected this parameter in PO and Aceh bulls exclusively (p<0.05). Consistently and significantly, PRM1 was positively correlated with the PM and viability of sperm and negatively associated with its DNA fragmentation in LIM, FH, PO, and Aceh bulls (p<0.05; p<0.01). The correlation analysis between PRM2 and PRM3 and semen quality parameters varied across all bull breeds; some were positively and negatively correlated (p<0.05; p<0.01), and some were not correlated at all.
conclusionPRM1 has excellent potential as a protein marker of semen production and quality in bulls at the National AI Center of Indonesia.
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