ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2022
Translational demand is not a major source of plasmid-associated fitness costs.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Invasive plasmids as ecosystem engineers-from mechanism to application.Essays in biochemistry · 2026Review
- The leading region of many plasmids is adapted for translational efficiency.Nucleic acids research · 2026Article
- The plasmid-host fitness landscape: a new paradigm for predicting the fate of mobile resistance.Applied and environmental microbiology · 2026Review
- Genomic characterization of vancomycin-resistantFrontiers in microbiology · 2026Article
- Intracellular competition shapes plasmid population dynamics.Science (New York, N.Y.) · 2025Article
- Dual-promoter systems drive plasmid loss via non-translational burden in geraniol-producing E. coli.Archives of microbiology · 2025Article
- Genetic compatibility and ecological connectivity drive the dissemination of antibiotic resistance genes.Nature communications · 2025Article
- Unravelling Prokaryotic Codon Usage: Insights from Phylogeny, Influencing Factors and Pathogenicity.Current genomics · 2025Review
- A plasmid-chromosome crosstalk in multidrug resistant enterobacteria.Nature communications · 2024Article
- The evolutionary landscape of prokaryotic chromosome/plasmid balance.Communications biology · 2024Article
- Specific codons control cellular resources and fitness.Science advances · 2024Article
- A mathematician's guide to plasmids: an introduction to plasmid biology for modellers.Microbiology (Reading, England) · 2023Review
- Plasmid Costs Explain Plasmid Maintenance, Irrespective of the Nature of Compensatory Mutations.Antibiotics (Basel, Switzerland) · 2023Article
- Vertical and horizontal gene transfer tradeoffs direct plasmid fitness.Molecular systems biology · 2023Article
- Physiological Responses of Ribosomal Protein S12 K43 Mutants of Corynebacterium glutamicum.Current microbiology · 2022Article
- Translational demand is not a major source of plasmid-associated fitness costs.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Introduction: the secret lives of microbial mobile genetic elements.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Emergence of a High-RiskFrontiers in microbiology · 2022Article
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Authors and funding
7 authors at 2 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasmids are key drivers of bacterial evolution because they are crucial agents for the horizontal transfer of adaptive traits, such as antibiotic resistance. Most plasmids entail a metabolic burden that reduces the fitness of their host if there is no selection for plasmid-encoded genes. It has been hypothesized that the translational demand imposed by plasmid-encoded genes is a major mechanism driving the fitness cost of plasmids. Plasmid-encoded genes typically present a different codon usage from host chromosomal genes. As a consequence, the translation of plasmid-encoded genes might sequestrate ribosomes on plasmid transcripts, overwhelming the translation machinery of the cell. However, the pervasiveness and origins of the translation-derived costs of plasmids are yet to be assessed. Here, we systematically altered translation efficiency in the host cell to disentangle the fitness effects produced by six natural antibiotic resistance plasmids. We show that limiting translation efficiency either by reducing the number of available ribosomes or their processivity does not increase plasmid costs. Overall, our results suggest that ribosomal paucity is not a major contributor to plasmid fitness costs. This article is part of the theme issue 'The secret lives of microbial mobile genetic elements'.
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