ArticleFunctional & integrative genomics2022
Identification and bioinformatics analysis of differentially expressed milk exosomal microRNAs in milk exosomes of heat-stressed Holstein cows.
Article in Functional & integrative genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Heat Stress Effects on Milk Production and the Genomic Architecture of Thermotolerance in Dairy Cattle.Biology · 2026Review
- Investigating milk-derived extracellular vesicles as mediators of maternal stress and environmental intervention.Molecular psychiatry · 2026Article
- Article
- Milk-Derived EVs from Different Animal Sources: An Overview on Their Detection, Isolation and Pleiotropic Exerted Effects.International journal of molecular sciences · 2026Review
- Investigating milk-derived extracellular vesicles as mediators of maternal stress and environmental intervention.bioRxiv : the preprint server for biology · 2025Article
- Proteomic Analysis of Differentially Expressed Plasma Exosome Proteins in Heat-StressedAnimals : an open access journal from MDPI · 2025Article
- Rumen-mammary gland axis and bacterial extracellular vesicles: Exploring a new perspective on heat stress in dairy cows.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Review
- Comparison of colostrum and milk extracellular vesicles small RNA cargo in water buffalo.Scientific reports · 2024Article
- Recent insights into breast milk microRNA: their role as functional regulators.Frontiers in nutrition · 2024Review
- Small RNA sequencing analysis of exosomes derived from umbilical plasma in IUGR lambs.Communications biology · 2023Article
- miR-27a-3p relieves heat stress-induced mitochondrial damage and aberrant milk protein synthesis through MEK/ERK pathway in BMECs.Cell stress & chaperones · 2023Article
- Regulatory Role of microRNA of Milk Exosomes in Mastitis of Dairy Cows.Animals : an open access journal from MDPI · 2023Article
- Comprehensive Profiling of ceRNA (circRNA-miRNA-mRNA) Networks in Hypothalamic-Pituitary-Mammary Gland Axis of Dairy Cows under Heat Stress.International journal of molecular sciences · 2023Article
- Extracellular Vesicles from Animal Milk: Great Potentialities and Critical Issues.Animals : an open access journal from MDPI · 2022Review
- Exosomes in Mastitis-Research Status, Opportunities, and Challenges.Animals : an open access journal from MDPI · 2022Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
In summer, heat stress is one of the primary reasons for the compromised health and low milk productivity of dairy cows. Hyperthermia affects milk synthesis and secretion in the mammary glands of dairy cows. As molecules for intercellular communication, milk-derived exosomes carry genetic material, proteins, and lipids, playing a crucial role in mammary tissue growth and milk synthesis in dairy cows. The aim of this study was to explore the milk exosomal miRNA profile of heat-stressed and normal Holstein cows. We isolated and identified milk exosomes to screening for differentially expressed miRNAs using small RNA sequencing. Then, TargetScan and miRanda algorithms were used to predict the putative targets of the differentially expressed miRNAs, whereas GO and KEGG pathway enrichment analyses were performed for the differentially expressed miRNA-target genes. Our results showed that 215 miRNAs were significantly differentially expressed in heat-stressed milk exosomes, of which one was upregulated and 214 were significantly downregulated. GO and KEGG enrichment analyses indicated that differentially expressed miRNAs might play a role in apoptosis, autophagy, and the p38 MAPK pathway. qRT-PCR assay verified that the expression of miRNAs was consistent with the sequencing results, warranting further verification of their specific targets of action. In conclusion, changes in the miRNA expression profile of milk exosomes indicated the role of exosomal miRNAs in regulating heat stress resistance and apoptosis in dairy cows. Our results suggested that milk-derived exosomal miRNAs could increase mammary gland resistance to heat stress, thereby enhancing milk synthesis in dairy cows.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.