Evidence map›Paper›PMID 34837712›Full record

ArticleJournal of clinical laboratory analysis2022

Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS-CoV-2 as assessed by a plaque assay.

Kyoko Hayashi, Satomi Asai, Kazuo Umezawa, Hidehumi Kakizoe, Hayato Miyachi, Masanobu Morita, Takaaki Akaike, Hitoshi Kuno, Satoko Komatsu, Takumi Watanabe and 1 more

Abstract read
In one paragraph

Article in Journal of clinical laboratory analysis, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Marine drugs · 2023
    Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Kyoko HayashiCollege of Life and Health Sciences, Chubu University, Kasugai, Japan.ORCID https://orcid.org/0000-0002-9582-1201
Satomi AsaiDepartment of laboratory Medicine, School of Medicine, Tokai University, Isehara, Japan.
Kazuo UmezawaDepartment of Emergency and Critical Care, School of Medicine, Tokai University, Isehara, Japan.
Hidehumi KakizoeDepartment of laboratory Medicine, School of Medicine, Tokai University, Isehara, Japan.
Hayato MiyachiDepartment of laboratory Medicine, School of Medicine, Tokai University, Isehara, Japan.ORCID https://orcid.org/0000-0002-5343-6751
Masanobu MoritaGraduate School of Medicine, Tohoku University, Sendai, Japan.
Takaaki AkaikeGraduate School of Medicine, Tohoku University, Sendai, Japan.
Hitoshi KunoDENSO Corporation, Kariya, Japan.
Satoko KomatsuDENSO Corporation, Kariya, Japan.
Takumi WatanabeCollege of Life and Health Sciences, Chubu University, Kasugai, Japan.
Toshio KawaharaCollege of Life and Health Sciences, Chubu University, Kasugai, Japan.

Funding

JST Adaptable and Seamless Technology Transfer Program through Target-driven R & D JPMJTR204H
6 · The paper itself

Abstract

backgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19) and is capable of human-to-human transmission and rapid global spread. Thus, the establishment of high-quality viral detection and quantification methods, and the development of anti-SARS-CoV-2 agents are critical.

methodsHere, we present the rapid detection of infectious SARS-CoV-2 particles using a plaque assay with 0.5% agarose-ME (Medium Electroosmosis) as an overlay medium.

resultsThe plaques were capable of detecting the virus within 36-40 h post-infection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga (Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS-CoV-2 in a time- and concentration-dependent manner.

conclusionsThese results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS-CoV-2.

Indexed as

AnimalsAntiviral AgentsChlorocebus aethiopsChlorophytaCOVID-19GalactoseGlyceridesHumansMicroalgaeSARS-CoV-2Vero CellsViral Plaque AssayAntiviral AgentsGalactoseGlyceridesmonogalactosyl diacylglyceridedetection timeinfectious virusnatural productquantificationvirucidal action

Identifiers

PMID34837712
PMCPMC8761427

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.