ArticleMolecular biology reports2022
Effects of valerate on intestinal barrier function in cultured Caco-2 epithelial cell monolayers.
Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Early growth restriction disrupts mice intestinal clock and homeostasis without being prevented by lactoferrin.Pediatric research · 2026Article
- Effects ofAnimals : an open access journal from MDPI · 2026Article
- Members of Lachnospiraceae produce valerate and caproate in response to short-chain fatty acids.Microbiome · 2025Article
- The role of fecal short-chain fatty acids in neonatal necrotizing enterocolitis.Translational pediatrics · 2025Article
- Optimizing Extracellular Products from Vibrio proteolyticus for Their Use as Postbiotics in Aquaculture.Marine biotechnology (New York, N.Y.) · 2025Article
- Vancomycin-resistant enterococci utilise antibiotic-enriched nutrients for intestinal colonisation.Nature communications · 2025Article
- Exploring the role of gut microbiota in rheumatoid arthritis: the effects of diet and drug supplementation.BMC rheumatology · 2025Article
- The emerging role of microbiota derived SCFAs in neurodegenerative disorders.Brain, behavior, & immunity - health · 2025Review
- Role of Gut Microbial Metabolites in Ischemic and Non-Ischemic Heart Failure.International journal of molecular sciences · 2025Review
- Improved quality of cottonseed meal: effect of cottonseed protein isolate on growth performance, nutrient digestibility, and intestinal health in growing pigs.Journal of animal science · 2025Article
- Evaluation of the Bioavailability of Iodine and Arsenic in Raw and CookedFoods (Basel, Switzerland) · 2024Article
- Ningxiang Pig-DerivedAnimals : an open access journal from MDPI · 2024Article
- No Impact of Enteral Nutrition on Fecal Short-Chain Fatty Acids in Children with Cerebral Palsy.Biomedicines · 2024Article
- Article
- Citrus Extract High in Flavonoids Beneficially Alters Intestinal Metabolic Responses in Subjects with Features of Metabolic Syndrome.Foods (Basel, Switzerland) · 2023Article
- Antibiotics promote intestinal growth of carbapenem-resistant Enterobacteriaceae by enriching nutrients and depleting microbial metabolites.Nature communications · 2023Article
- Role of the gut microbiota in nutrient competition and protection against intestinal pathogen colonization.Microbiology (Reading, England) · 2023Review
- Colonization and development of the gut microbiome in calves.Journal of animal science and biotechnology · 2023Review
- Human Adult Microbiota in a Static Colon Model: AhR Transcriptional Activity at the Crossroads of Host-Microbe Interaction.Foods (Basel, Switzerland) · 2022Article
- Effects of high-altitude hypoxic environment on colonic inflammation, intestinal barrier and gut microbiota in three-way crossbred commercial pigs.Frontiers in microbiology · 2022Article
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundShort-chain fatty acids (SCFAs) are a group of microbial metabolites of undigested dietary fiber, protein and unabsorbed amino acids in the colon, well-known for their gut health promoting benefits. A relatively high intestinal level of valerate was found in the healthy human subjects. However, the intestinal protection effects and the underlying mechanism of valerate are waiting to be verified and elucidated. METHODS AND
resultsIn the present study, valerate, a SCFAs mainly converted from proteins or amino acids, was demonstrated to promote intestinal barrier function at its physiological concentrations of 0-4 mM in the Caco-2 cell monolayer model of intestinal barrier using transepithelial electrical resistance (TEER) assay and paracellular permeability assay. Valerate achieved the maximum increase in the TEER at 2 mM and reduced the paracellular permeability. Its intestinal barrier function promoting activity is similar to that of butyrate, with a broader range of effective concentrations than the later. Through western blot analysis, this activity is linked to the valerate-induced AMPK activation and tight junctions (TJs) assembly, but not to the reinforced expression of TJs related proteins.
conclusionsIt provides direct experimental evidence supporting valerate's function in intestinal health, implying the once under-valued function of valerate and its amino acid precursors. The valerate's role in regulating intestine homeostasis and its possible synergetic effects with other SCFAs warranted to be further investigated.
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