Rapid and sensitive detection of Karenia mikimotoi by loop-mediated isothermal amplification combined with a lateral flow dipstick.

Xiaotian Han, Ting Zhao, Tian Yan, Rencheng Yu

Abstract read

In one paragraph

Article in Environmental science and pollution research international, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.6field-weighted citation impact, top 35% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

Development of a rapid detection method forFrontiers in microbiology · 2023
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Xiaotian HanChangjiang River Estuary Ecosystem Research Station, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, Shandong, China. xthan@qdio.ac.cn.ORCID http://orcid.org/0000-0003-1175-7925

Ting Zhao, Guangzhou, China.

Tian YanChangjiang River Estuary Ecosystem Research Station, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, Shandong, China.

Rencheng YuChangjiang River Estuary Ecosystem Research Station, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, Shandong, China.

Chinese Academy of Sciences · CN

Funding

Aoshan Science and technology innovation project, Qingdao National Laboratory for Marine Science and Technology Grant No. 2016ASKJ02-1Foundation of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms Grant No. MATHAB201707National Key Research and Development Program of China(grant No. 2017YFC1404304) Grant No. 2017YFC1404304Sino-Australian Centre for Healthy Coasts Grant No. 2016YFE0101500Special Foundation for National Science and Technology Basic Research Program of China Grant No. 2018FY100207Strategic Priority Research Program of the Chinese Academy of Sciences Grant No. XDA23050302

6 · The paper itself

Abstract

Harmful algal blooms frequently occur in various coastal regions worldwide, deteriorating marine ecology and causing huge economic losses. Therefore, developing a potential method for rapid detection of harmful algae species is highly necessitated. In this study, a loop-mediated isothermal amplification (LAMP) method coupled with a lateral flow dipstick (LFD) was developed for detecting the harmful algae Karenia mikimotoi. In this method, the internal transcribed spacer (ITS) sequence of K. mikimotoi was used as the template, and the corresponding specific primers were designed by the online software PrimerExplorer V5. Biotin was labeled on the 5' end of forward inner primer (FIP), and the LAMP reaction was performed under the determined optimal conditions of 63℃ and 60 min. The lowest concentration of K. mikimotoi DNA tested using LAMP was 3.3 × 10

Indexed as

DinoflagellidaNucleic Acid Amplification TechniquesMolecular Diagnostic TechniquesSensitivity and SpecificityKarenia mikimotoiLateral flow dipstick (LFD)Loop-mediated isothermal amplification (LAMP)Rapid detection

Identifiers

PMID34837104

DOI10.1007/s11356-021-17536-w

OpenAlexW3216130869

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.