ArticleBMC genomics2021
Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change.
Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Horizon scanning of potential environmental applications of terrestrial animals, fish, algae and microorganisms produced by genetic modification, including the use of new genomic techniques.Frontiers in genome editing · 2024Pooled it
- Influence of MC1R Gene Variants on Coat Color of Indicine Cattle Breeds.Animal genetics · 2026Article
- Clinical outcome of an SCNT-derivedFrontiers in genome editing · 2026Article
- Physiological Responses and Pregnancy Rate of Black and White Holstein Heifers During Hot and Cold Seasons in a Desert Region.Animals : an open access journal from MDPI · 2025Article
- Article
- Ruminant livestock farmers and industry are leading innovation to deliver human nutrition and improved environmental outcomes through sector lifecycle collaboration: a review of case studies.Animal frontiers : the review magazine of animal agriculture · 2025Article
- An overview of recent technological developments in bovine genomics.Veterinary and animal science · 2024Review
- Current status and future of gene engineering in livestock.BMB reports · 2024Review
- Coat Color in Local Goats: Influence on Environmental Adaptation and Productivity, and Use as a Selection Criterion.Biology · 2023Review
- Heat stress in horses: a literature review.International journal of biometeorology · 2023Review
- Analysis of runs of homozygosity of cattle living in different climate zones.Journal of animal science · 2023Article
- Candidate genes associated with heat stress and breeding strategies to relieve its effects in dairy cattle: a deeper insight into the genetic architecture and immune response to heat stress.Frontiers in veterinary science · 2023Review
- PMEL p.Leu18del dilutes coat color of Kumamoto sub-breed of Japanese Brown cattle.BMC genomics · 2022Article
- Towards progressive regulatory approaches for agricultural applications of animal biotechnology.Transgenic research · 2022Review
- Cytoplasmic Injection of Zygotes to Genome Edit Naturally Occurring Sequence Variants Into Bovine Embryos.Frontiers in genetics · 2022Article
- Improving Genomic Selection for Heat Tolerance in Dairy Cattle: Current Opportunities and Future Directions.Frontiers in genetics · 2022Review
- The genomes of precision edited cloned calves show no evidence for off-target events or increased de novo mutagenesis.BMC genomics · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHigh-producing Holstein Friesian dairy cattle have a characteristic black and white coat, often with large proportions of black. Compared to a light coat color, black absorbs more solar radiation which is a contributing factor to heat stress in cattle. To better adapt dairy cattle to rapidly warming climates, we aimed to lighten their coat color by genome editing.
resultsUsing gRNA/Cas9-mediated editing, we introduced a three bp deletion in the pre-melanosomal protein 17 gene (PMEL) proposed as causative variant for the semi-dominant color dilution phenotype observed in Galloway and Highland cattle. Calves generated from cells with homozygous edits revealed a strong color dilution effect. Instead of the characteristic black and white markings of control calves generated from unedited cells, the edited calves displayed a novel grey and white coat pattern.
conclusionThis, for the first time, verified the causative nature of the PMEL mutation for diluting the black coat color in cattle. Although only one of the calves was healthy at birth and later succumbed to a naval infection, the study showed the feasibility of generating such edited animals with the possibility to dissect the effects of the introgressed edit and other interfering allelic variants that might exist in individual cattle and accurately determine the impact of only the three bp change.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.