Evidence map›Paper›PMID 34834948›Full record

ArticleViruses2021

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021.

Dong-Hun Lee, Zeinab H Helal, Junwon Kim, Amelia Hunt, Alyza Barbieri, Natalie Tocco, Salvatore Frasca, Kirklyn Kerr, Ji-Yeon Hyeon, David H Chung and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. SeronegativeVeterinary sciences · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Dong-Hun LeeDepartment of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA.ORCID 0000-0002-0820-7378
Zeinab H HelalDepartment of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA.ORCID 0000-0002-7869-9806
Junwon KimDepartment of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA.
Amelia HuntDepartment of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA.
Alyza BarbieriConnecticut Veterinary Medical Diagnostic Laboratory, Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA.
Natalie ToccoDepartment of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA.
Salvatore FrascaDepartment of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA.
Kirklyn KerrDepartment of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA.
Ji-Yeon HyeonDepartment of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA.
David H ChungDepartment of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA.
Guillermo RisattiDepartment of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06268, USA.

Funding

FDA HHS U18 FD004640University of Connecticut College of Agriculture, Health and Natural Resources
6 · The paper itself

Abstract

We report the first detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a 3-month-old dog in Connecticut that died suddenly and was submitted to the state veterinary diagnostic laboratory for postmortem examination. Viral RNA was detected in multiple organs of the dog by reverse transcription real time-PCR (RT-qPCR). Negative and positive sense strands of viral RNA were visualized by in situ hybridization using RNAscope technology. Complete genome sequencing and phylogenetic analysis of the hCoV-19/USA/CT-CVMDL-Dog-1/2021 (CT_Dog/2021) virus were conducted to identify the origin and lineage of the virus. The CT_Dog/2021 virus belonged to the GH/B1.2. genetic lineage and was genetically similar to SARS-CoV-2 identified in humans in the U.S. during the winter of 2020-2021. However, it was not related to other SARS-CoV-2 variants identified from companion animals in the U.S. It contained both the D614G in spike and P323L in nsp12 substitutions, which have become the dominant mutations in the United States. The continued sporadic detections of SARS-CoV-2 in companion animals warrant public health concerns about the zoonotic potential of SARS-CoV-2 and enhance our collective understanding of the epidemiology of the virus.

Indexed as

AnimalsConnecticutCOVID-19COVID-19 Nucleic Acid TestingDogsFemaleHumansMutationPhylogenyRNA, ViralSARS-CoV-2Whole Genome SequencingRNA, ViralConnecticutCOVID-19dogepidemiologyRNAscopeSARS-CoV-2sudden death

Identifiers

PMID34834948
PMCPMC8623824

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.