ArticleViruses2021
Metagenomic Identification of Viral Sequences in Laboratory Reagents.
Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- Evolving strategies for virus discovery.Microbial genomics · 2026Review
- Empowering One Health with metagenomics.One health outlook · 2026Review
- Microbial cell-free DNA for rapid pathogen identification in clinical diagnostics: a proof of concept study.Genome medicine · 2026Article
- CRESSENT: a bioinformatics toolkit to explore and improve ssDNA virus annotation.Microbial genomics · 2026Article
- Unveiling pathogens and contaminants: refining metagenomics for clinical diagnostics.Frontiers in microbiology · 2026Article
- Detection of viral sequences at single-cell resolution identifies novel viruses associated with host gene expression changes.Nature biotechnology · 2026Article
- Targeted decontamination of sequencing data with CLEAN.NAR genomics and bioinformatics · 2025Article
- Negativeome characterization and decontamination in early-life virome studies.Nature communications · 2025Article
- Identification of shared viral sequences in peat moss metagenomes reveals elements of a possible Sphagnum core virome.Environmental microbiome · 2025Article
- Wastewater Metavirome Diversity: Exploring Replicate Inconsistencies and Bioinformatic Tool Disparities.International journal of environmental research and public health · 2025Article
- Efficient and accurate detection of viral sequences at single-cell resolution reveals putative novel viruses perturbing host gene expression.bioRxiv : the preprint server for biology · 2025Article
- Improving the reporting of metagenomic virome-scale data.Communications biology · 2024Article
- A metagenomic investigation of the faecal RNA virome structure of asymptomatic chickens obtained from a commercial farm in Durban, KwaZulu-Natal province, South Africa.BMC genomics · 2024Article
- The virome of the kitome: small circular virus-like genomes in laboratory reagents.Microbiology resource announcements · 2024Article
- Diverse Small Circular DNA Viruses Identified in an American Wigeon Fecal Sample.Microorganisms · 2024Article
- Screening for atypical porcine pestivirus in Swedish boar semen used for artificial insemination and a characterisation of the seminal RNA microbiome including the virome.BMC veterinary research · 2023Article
- Unmasking the tissue-resident eukaryotic DNA virome in humans.Nucleic acids research · 2023Article
- RAPIDViruses · 2023Article
- From Gut to Blood: Spatial and Temporal Pathobiome Dynamics during Acute Abdominal Murine Sepsis.Microorganisms · 2023Article
- Human Clinical Isolates of Pathogenic Fungi Are Host to Diverse Mycoviruses.Microbiology spectrum · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metagenomic next-generation sequencing has transformed the discovery and diagnosis of infectious disease, with the power to characterise the complete 'infectome' (bacteria, viruses, fungi, parasites) of an individual host organism. However, the identification of novel pathogens has been complicated by widespread microbial contamination in commonly used laboratory reagents. Using total RNA sequencing ("metatranscriptomics") we documented the presence of contaminant viral sequences in multiple 'blank' negative control sequencing libraries that comprise a sterile water and reagent mix. Accordingly, we identified 14 viral sequences in 7 negative control sequencing libraries. As in previous studies, several circular replication-associated protein encoding (CRESS) DNA virus-like sequences were recovered in the blank control libraries, as well as contaminating sequences from the
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.