Evidence map›Paper›PMID 34834920›Full record

ArticleViruses2021

A CRISPR Activation Screen Identifies an Atypical Rho GTPase That Enhances Zika Viral Entry.

Anh Phuong Luu, Zhenlan Yao, Sangeetha Ramachandran, Stephanie A Azzopardi, Linde A Miles, William M Schneider, H-Heinrich Hoffmann, Leonia Bozzacco, Gustavo Garcia, Danyang Gong and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Functional Roles and Host Interactions ofPathogens (Basel, Switzerland) · 2025
    Review
  6. Review
  7. Article
  8. Atypical RhoUV GTPases in development and disease.Biochemical Society transactions · 2024
    Review
  9. Article
  10. Article
  11. Review
  12. Review
  13. Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 6 institutions in 1 country.

Anh Phuong LuuDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Zhenlan YaoDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Sangeetha RamachandranDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Stephanie A AzzopardiLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
Linde A MilesHuman Oncology & Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.ORCID 0000-0003-3578-2842
William M SchneiderLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
H-Heinrich HoffmannLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.ORCID 0000-0003-0554-0244
Leonia BozzaccoLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
Gustavo GarciaDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.ORCID 0000-0002-1649-9251
Danyang GongDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Robert DamoiseauxDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.ORCID 0000-0002-7611-7534
Hengli TangDepartment of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
Kouki MorizonoDivision of Hematology and Oncology, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
Charles M RudinDruckenmiller Center for Lung Cancer Research and Department of Medicine, Thoracic Oncology Service, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.ORCID 0000-0001-5204-3465
Ren SunDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
Vaithilingaraja ArumugaswamiDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
John T PoirierLaura and Isaac Perlmutter Cancer Center, New York University Langone Health, New York, NY 10016, USA.
Margaret R MacDonaldLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.ORCID 0000-0001-5177-2068
Charles M RiceLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
Melody M H LiDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
University of California, Los Angeles · USRockefeller University · USMemorial Sloan Kettering Cancer Center · USCalifornia NanoSystems Institute · USFlorida State University · USNYU Langone Health · US

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Hongbiao Carl Lekaye · 1985 to 2026
$347.4M
Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5M
Virology CoreP30AI028697 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KITCHEN, SCOTT G · 1991 to 2018
$39.9M
Viral Immunology CoreU19AI149504 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHEN, IRVIN S.Y., KITCHEN, SCOTT G · 2020 to 2024
$14.3M
Type I interferon-stimulated genes and the antiviral immune responseR01AI091707 · NIAID · ROCKEFELLER UNIVERSITY · PI RICE, CHARLES M · 2011 to 2020
$5.5M
B-cell-specific transduction for anti-HIV antibody and B-cell receptor expressionR01AI145044 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MORIZONO, KOKI · 2019 to 2023
$1.9M
Functional analysis of host and viral determinants for ZAP inhibitionR01AI158704 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LI, MELODY · 2021 to 2025
$1.9M
National Institute of Allergy and Infectious Diseases R01AI158704NCI NIH HHS P30 CA008748NCI NIH HHS P30 CA016042NIAID NIH HHS P30 AI028697NIAID NIH HHS R01 AI091707NIAID NIH HHS R01 AI145044NIAID NIH HHS R01 AI158704NIAID NIH HHS U19 AI149504
6 · The paper itself

Abstract

Zika virus (ZIKV) is a re-emerging flavivirus that has caused large-scale epidemics. Infection during pregnancy can lead to neurologic developmental abnormalities in children. There is no approved vaccine or therapy for ZIKV. To uncover cellular pathways required for ZIKV that can be therapeutically targeted, we transcriptionally upregulated all known human coding genes with an engineered CRISPR-Cas9 activation complex in human fibroblasts deficient in interferon (IFN) signaling. We identified Ras homolog family member V (

Indexed as

A549 CellsCRISPR-Cas SystemsGTP-Binding ProteinsHumansNeoplasm Proteinsp21-Activated KinasesrhoB GTP-Binding ProteinTranscriptional Coactivator with PDZ-Binding Motif ProteinsVirus InternalizationVirus ReplicationZika VirusZika Virus InfectionGTP-Binding ProteinsNeoplasm Proteinsp21-Activated KinasesPAK1 protein, humanrhoB GTP-Binding ProteinRHOB protein, humanRHOV protein, humanTranscriptional Coactivator with PDZ-Binding Motif ProteinsWWTR1 protein, humanCRISPR activationproviral factorsRho GTPasesRhoVWWTR1Zika virus

Identifiers

PMID34834920
PMCPMC8623001
OpenAlexW3208918800

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.