Evidence map›Paper›PMID 34834753›Full record

ArticlePlants (Basel, Switzerland)2021

Identification and Characterization of Citrus Concave Gum-Associated Virus Infecting Citrus and Apple Trees by Serological, Molecular and High-Throughput Sequencing Approaches.

Maria Minutolo, Maria Cinque, Michela Chiumenti, Francesco Di Serio, Daniela Alioto, Beatriz Navarro

Open access · goldAbstract read
In one paragraph

Article in Plants (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.5field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Maria MinutoloDipartimento di Agraria, Università degli Studi di Napoli Federico II, 80055 Portici, Italy.ORCID 0000-0001-7816-2657
Maria CinqueDipartimento di Agraria, Università degli Studi di Napoli Federico II, 80055 Portici, Italy.ORCID 0000-0003-4459-9433
Michela ChiumentiIstituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, 70126 Bari, Italy.ORCID 0000-0002-8412-3037
Francesco Di SerioIstituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, 70126 Bari, Italy.ORCID 0000-0003-2822-704X
Daniela AliotoDipartimento di Agraria, Università degli Studi di Napoli Federico II, 80055 Portici, Italy.ORCID 0000-0002-4954-8363
Beatriz NavarroIstituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, 70126 Bari, Italy.ORCID 0000-0001-8981-1018
Institute for Sustainable Plant Protection · ITUniversity of Naples Federico II · IT

Funding

European Union 734736Regione Campania URCOFIRegione Puglia INNOCI
6 · The paper itself

Abstract

Citrus concave gum-associated virus (CCGaV) is a negative-stranded RNA virus, first reported a few years ago in citrus trees from Italy. It has been reported in apple trees in the USA and in Brazil, suggesting a wider host range and geographic distribution. Here, an anti-CCGaV polyclonal antiserum to specifically detect the virus has been developed and used in a standard double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) that has been validated as a sensitive and reliable method to detect this virus both in citrus and apple trees. In contrast, when the same antiserum was used in direct tissue-blot immunoassay, CCGaV was efficiently detected in citrus but not in apple. Using this antiserum, the first apple trees infected by CCGaV were identified in Italy and the presence of CCGaV in several apple cultivars in southern Italy was confirmed by field surveys. High-throughput sequencing (HTS) allowed for the assembling of the complete genome of one CCGaV Italian apple isolate (CE-c3). Phylogenetic analysis of Italian CCGaV isolates from apple and citrus and those available in the database showed close relationships between the isolates from the same genus (

Indexed as

apple rubbery wood virus 2ARWV-2CCGaVcoguvirusconcave gum diseaseDAS-ELISADTBIAnext-generation sequencingvirus detection

Identifiers

PMID34834753
PMCPMC8625769
OpenAlexW3211392336

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.