ArticleCells2021
Next- and Third-Generation Sequencing Outperforms Culture-Based Methods in the Diagnosis of Ascitic Fluid Bacterial Infections of ICU Patients.
Article in Cells, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Altered Pathogen Spectrum of Spontaneous Bacterial Peritonitis in Patients Treated With Proton Pump Inhibitors.Alimentary pharmacology & therapeutics · 2026Article
- Next-generation sequencing of cell-free microbial DNA in blood samples of critically ill children: a single-center experience.Molecular and cellular pediatrics · 2026Article
- Nanopore-targeted sequencing improves the diagnosis and treatment of patients with infectious ascites.Journal of translational medicine · 2026Article
- Lower airway microbiota compositions and diversity among ventilator-associated pneumonia patients across COVID-19 epidemic phases: a retrospective study.Microbiology spectrum · 2025Article
- Finegoldia magna infection after lumbar spine surgery determined based on targeted next-generation sequencing.BMC musculoskeletal disorders · 2025Article
- Diagnostic performance of broad-range PCR in bacterial peritonitis.Frontiers in cellular and infection microbiology · 2025Article
- Next-generation sequencing guides the treatment of severe community-acquired pneumonia with empiric antimicrobial therapy failure: A propensity-score-matched study.PLoS neglected tropical diseases · 2024Article
- 16S rRNA Next-Generation Sequencing May Not Be Useful for Examining Suspected Cases of Spontaneous Bacterial Peritonitis.Medicina (Kaunas, Lithuania) · 2024Article
- Enhancing ascitic fungal infection diagnosis through next-generation sequencing: a pilot study in surgical ICU patients.Frontiers in cellular and infection microbiology · 2024Article
- Soft tissue tuberculosis detected by next-generation sequencing: A case report and review of literature.World journal of clinical cases · 2023Article
- Detection ofOpen life sciences · 2023Article
- Detection of Pathogens and Antimicrobial Resistance Genes in Ventilator-Associated Pneumonia by Metagenomic Next-Generation Sequencing Approach.Infection and drug resistance · 2023Article
- Bloodstream infection, peritonitis, and pneumonia caused byFrontiers in cellular and infection microbiology · 2023Review
- Community-acquired pneumonia in Korean children: time to read between the lines.Clinical and experimental pediatrics · 2023Article
- Article
- "Bacterial Consortium": A Potential Evolution of Fecal Microbiota Transplantation for the Treatment ofBioMed research international · 2022Article
- The Role of Gut Microbiota in Some Liver Diseases: From an Immunological Perspective.Frontiers in immunology · 2022Review
- Next Generation Sequencing Based Detection of Bacterial Species in Bile After Liver Transplantation. Hope, Hype or Hyperbole?Journal of clinical and experimental hepatologyArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
objectivesInfections of the ascitic fluid are serious conditions that require rapid diagnosis and treatment. Ascites is often accompanied by other critical pathologies such as gastrointestinal bleeding and bowel perforation, and infection increases the risk of mortality in intensive care patients. Owing to a relatively low success rate of conventional culture methods in identifying the responsible pathogens, new methods may be helpful to guide antimicrobial therapy and to refine empirical regimens. Here, we aim to assess outcomes and to identify responsible pathogens in ascitic fluid infections, in order to improve patients' care and to guide empirical therapy.
methodsBetween October 2019 and March 2021, we prospectively collected 50 ascitic fluid samples from ICU patients with suspected infection. Beside standard culture-based microbiology methods, excess fluid underwent DNA isolation and was analyzed by next- and third-generation sequencing (NGS) methods.
resultsNGS-based methods had higher sensitivity in detecting additional pathogenic bacteria such as
conclusionsOur results show that, in ascitic fluid infections, NGS-based methods have a higher sensitivity for the identification of clinically relevant pathogens than standard microbiological culture diagnostics, especially in detecting hard-to-culture anaerobic bacteria. Patients with such infections may benefit from the use of NGS methods by the possibility of earlier and better targeted antimicrobial therapy, which has the potential to lower the high morbidity and mortality in critically ill patients with ascitic bacterial infection.
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