Evidence map›Paper›PMID 34831046›Full record

ArticleCells2021

Comparison of Three CD3-Specific Separation Methods Leading to Labeled and Label-Free T Cells.

Ronald Weiss, Wilhelm Gerdes, Rommy Berthold, Ulrich Sack, Ulrike Koehl, Sunna Hauschildt, Anja Grahnert

Open access · goldAbstract readComparative Study
In one paragraph

Article in Cells, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.6field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. CAR-T cells immunotherapy in the treatment of glioblastoma.Cancer immunology, immunotherapy : CII · 2025
    Review
  3. CAR T Cell Nanosymbionts: Revealing the Boundless Potential of a New Dyad.International journal of molecular sciences · 2024
    Review
  4. Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Ronald WeissInstitute of Clinical Immunology, Medical Faculty, Leipzig University, Johannisallee 30, 04103 Leipzig, Germany.
Wilhelm GerdesCell.Copedia GmbH, Bosestrasse 4, 04109 Leipzig, Germany.
Rommy BertholdCell.Copedia GmbH, Bosestrasse 4, 04109 Leipzig, Germany.
Ulrich SackInstitute of Clinical Immunology, Medical Faculty, Leipzig University, Johannisallee 30, 04103 Leipzig, Germany.ORCID 0000-0002-7813-0492
Ulrike KoehlInstitute of Clinical Immunology, Medical Faculty, Leipzig University, Johannisallee 30, 04103 Leipzig, Germany.
Sunna HauschildtInstitute of Biology, Faculty of Life Sciences, Leipzig University, Talstrasse 33, 04103 Leipzig, Germany.
Anja GrahnertInstitute of Clinical Immunology, Medical Faculty, Leipzig University, Johannisallee 30, 04103 Leipzig, Germany.ORCID 0000-0001-5239-5735
Leipzig University · DEMedizinische Hochschule Hannover · DE

Funding

Development Bank of Saxony 100362591
6 · The paper itself

Abstract

T cells are an essential part of the immune system. They determine the specificity of the immune response to foreign substances and, thus, help to protect the body from infections and cancer. Recently, T cells have gained much attention as promising tools in adoptive T cell transfer for cancer treatment. However, it is crucial not only for medical purposes but also for research to obtain T cells in large quantities, of high purity and functionality. To fulfill these criteria, efficient and robust isolation methods are needed. We used three different isolation methods to separate CD3-specific T cells from leukocyte concentrates (buffy coats) and Ficoll purified PBMCs. To catch the target cells, the Traceless Affinity Cell Selection (TACS

Indexed as

Staining and LabelingCD3 ComplexCell CountCell ProliferationCell SeparationCell ShapeCell SurvivalCytokinesErythrocytesHumansT-LymphocytesCD3 ComplexCytokinesCD3isolationMACS®proliferationREAlease®separationTACS®T cells

Identifiers

PMID34831046
PMCPMC8616525
OpenAlexW3207726418

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.