ArticleJournal of clinical medicine2021
Establishing Irreversible Electroporation Electric Field Potential Threshold in A Suspension In Vitro Model for Cardiac and Neuronal Cells.
Article in Journal of clinical medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 46 citations in OpenAlex.
- Targeted ablation of epicardial ganglionated plexi during cardiac surgery with pulsed field electroporation (NEURAL AF).Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing · 2025Trial
- Computational Assessment of Electrical and Thermal Effects of Epicardial Pulsed Field Ablation Adjacent to Stented Arteries.Bioengineering (Basel, Switzerland) · 2026Article
- The Clinical Utility of 3D Electroanatomical Mapping for Atrial Fibrillation Ablation by Pulsed Field Ablation.Journal of arrhythmia · 2025Article
- Pulsed electric field ablation process-the effect of bifurcation stents on electric field and heat distribution.Scientific reports · 2025Article
- Leveraging electric current stimulation and chemical induction to enhance MSC differentiation into cardiomyocytes.Regenerative medicine · 2025Article
- Microsecond pulsed electric fields induce myocardial ablation by secondary mitochondrial damage and cell death mechanisms.Scientific reports · 2025Article
- Cardiac selectivity in pulsed field ablation.Current opinion in cardiology · 2025Review
- Pulsed electric field ablation process-a study of the effect of different shapes (materials) of metal stents on the ablation effect.Frontiers in cardiovascular medicine · 2025Article
- Feasibility study of intravascular pulsed electric field ablation for the treatment of cardiac arrhythmias.Frontiers in physiology · 2025Article
- Catheter ablation using pulsed-field energy: Do we finally have the magic wand to defeat atrial fibrillation?World journal of cardiology · 2024Article
- A New Hope for the Treatment of Atrial Fibrillation: Application of Pulsed-Field Ablation Technology.Journal of cardiovascular development and disease · 2024Review
- State-of-the-art pulsed field ablation for cardiac arrhythmias: ongoing evolution and future perspective.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2024Review
- Pulsed Field Energy in Atrial Fibrillation Ablation: From Physical Principles to Clinical Applications.Journal of clinical medicine · 2024Review
- Pulsed Field Ablation for Atrial Fibrillation: Mechanisms, Advantages, and Limitations.Reviews in cardiovascular medicine · 2024Review
- The Intrinsic Cardiac Nervous System: From Pathophysiology to Therapeutic Implications.Biology · 2024Review
- Opportunities and Challenges in Catheter-Based Irreversible Electroporation for Ventricular Tachycardia.Pathophysiology : the official journal of the International Society for Pathophysiology · 2024Review
- Pulsed Field Ablation and Neurocardiology: Inert to Efferents or Delayed Destruction?Reviews in cardiovascular medicine · 2024Article
- Epicardial pulsed-field ablation-impact of electric field and heat distribution induced by coronary metallic stents.Frontiers in cardiovascular medicine · 2024Article
- 'Single-shot' pulmonary vein isolation using a novel lotos pulsed field ablation catheter: a pre-clinical evaluation of feasibility, safety, and 30-day efficacy.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2023Article
- Cardioneuroablation Using Epicardial Pulsed Field Ablation for the Treatment of Atrial Fibrillation.Journal of cardiovascular development and disease · 2023Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
aimsIrreversible electroporation is an ablation technique being adapted for the treatment of atrial fibrillation. Currently, there are many differences reported in the in vitro and pre-clinical literature for the effective voltage threshold for ablation. The aim of this study is a direct comparison of different cell types within the cardiovascular system and identification of optimal voltage thresholds for selective cell ablation.
methodsMonophasic voltage pulses were delivered in a cuvette suspension model. Cell viability and live-dead measurements of three different neuronal lines, cardiomyocytes, and cardiac fibroblasts were assessed under different voltage conditions. The immediate effects of voltage and the evolution of cell death was measured at three different time points post ablation.
resultsAll neuronal and atrial cardiomyocyte lines showed cell viability of less than 20% at an electric field of 1000 V/cm when at least 30 pulses were applied with no significant difference amongst them. In contrast, cardiac fibroblasts showed an optimal threshold at 1250 V/cm with a minimum of 50 pulses. Cell death overtime showed an immediate or delayed cell death with a proportion of cell membranes re-sealing after three hours but no significant difference was observed between treatments after 24 h.
conclusionsThe present data suggest that understanding the optimal threshold of irreversible electroporation is vital for achieving a safe ablation modality without any side-effect in nearby cells. Moreover, the evolution of cell death post electroporation is key to obtaining a full understanding of the effects of IRE and selection of an optimal ablation threshold.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.